Inhibition of lipase activities by basic polysaccharide
Inhibition of lipase activities by basic polysaccharide
复制标题
DOI：
10.1194/jlr.m600258-jlr200
复制
发表时间：
2007-02-01
影响因子：
6.5
通讯作者：
Hiraki, Jun
中科院分区：
文献类型：
--
作者：
Tsujita, Takahiro;Takaichi, Hiroe;Hiraki, Jun
碱性多糖对胰脂肪酶和羧酸酯脂肪酶对磷脂酰胆碱和牛磺胆酸乳化的三油基甘油（TO）的水解均有较强的抑制作用。DEAE-Sephadex对胰脂肪酶和羧酸酯脂肪酶水解TO具有剂量依赖性；而羧甲基Sephadex和Sephadex G-50对水解无抑制作用。聚葡萄糖（PD）是一种可溶性多糖，是一种非常弱的胰脂肪酶抑制剂。然而，当一个碱性基团（一个DEAE基团）附着在PD上时，DEAE-PD对脂肪酶的抑制作用增加，这取决于DEAE基团的取代率。每个PD分子的正电荷数量在脂肪酶抑制中是重要的。与胡椒醚乙基和3-三乙基氨基-2-羟丙基等其他碱基也有类似的取代作用。天然碱性多糖壳聚糖对胰脂肪酶活性也有抑制作用。凝胶过滤实验表明DEAE-PD与胰脂肪酶结合不强。采用不同乳化剂研究DEAE-PD对胰脂肪酶水解TO的影响：DEAE-PD （50 mg/ml）对阿拉伯胶、磷脂酰丝氨酸和磷脂酸乳化的TO的水解无抑制作用。在体内，大鼠口服DEAE-PD降低了血浆中甘油三酯的峰值浓度，增加了粪脂排泄。这些结果表明，碱性多糖能够通过抑制胰腺脂肪酶活性来抑制小肠对膳食脂肪的吸收。——Tsujita， T.， H. Takaichi， T. Takaku， T. Sawai， N. Yoshida， J. Hiraki碱性多糖抑制脂肪酶活性的研究。
Basic polysaccharide strongly inhibited the hydrolysis of trioleoylglycerol (TO) emulsified with phosphatidylcholine and taurocholate by either pancreatic lipase or carboxylester lipase. DEAE-Sephadex dose-dependently inhibited the hydrolysis of TO by pancreatic lipase and carboxylester lipase; however, carboxymethyl-Sephadex and Sephadex G-50 did not inhibit the hydrolysis. Polydextrose (PD), a soluble polysaccharide, was a very weak inhibitor of pancreatic lipase. However, when a basic group, a DEAE group, was attached to PD, lipase inhibition by DEAE-PD was increased, and this was dependent on the substitution ratio of DEAE groups. The number of positive charges per PD molecule is important in lipase inhibition. Similar substitution effects were observed with other basic groups, such as piperidinoethyl and 3-triethylamino-2-hydroxypropyl. The natural basic polysaccharide, chitosan, also inhibited pancreatic lipase activity. Gel-filtration experiments suggested that DEAE-PD did not bind strongly to pancreatic lipase. The effect of DEAE-PD on TO hydrolysis by pancreatic lipase was studied using various emulsifiers: DEAE-PD (50 mg/ml) did not inhibit the hydrolysis of TO emulsified with arabic gum, phosphatidylserine, or phosphatidic acid. In vivo, oral administration of DEAE-PD to rats reduced the peak plasma triacylglycerol concentration and increased fecal lipid excretion. These results suggest that basic polysaccharide is able to suppress dietary fat absorption from the small intestine by inhibiting pancreatic lipase activity. - Tsujita, T., H. Takaichi, T. Takaku, T. Sawai, N. Yoshida, and J. Hiraki. Inhibition of lipase activities by basic polysaccharide.