Histopathological alterations of white seabass, Lates calcarifer, in acute and subchronic cadmium exposure
Histopathological alterations of white seabass, Lates calcarifer, in acute and subchronic cadmium exposure
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DOI：
10.1016/s0269-7491(02)00270-1
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发表时间：
2003-01-01
影响因子：
8.9
通讯作者：
Jaritkhuan, S
中科院分区：
文献类型：
--
作者：
Thophon, S;Kruatrachue, M;Jaritkhuan, S
采用光镜和扫描电镜研究了急性和亚慢性镉暴露对3月龄白鲈鱼、幼鱼的组织病理学改变。镉对钙化l的96 h LC50值为20.12 +/- 0.61 mg/l，最大可接受毒性浓度（MATC）为7.79 mg/l。鱼分别暴露于10 mg/l和0.8 mg/l Cd（以CdCl2H2O的形式）中96 h和90 d。研究表明，鳃片和肾小管是镉急性毒性作用的主要靶器官，而亚慢性暴露时，鳃的毒性作用小于肾脏和肝脏。鳃改变包括上皮细胞水肿伴柱细胞系统破裂，动脉瘤伴部分破裂，上皮细胞和氯细胞肥大和增生。肝窦充血，肝细胞水肿，空泡形成，暗色颗粒堆积。在亚慢性暴露的第二和第三个月观察肝细胞中的脂滴和糖原含量。肾小管细胞液泡肿胀，许多小管内积聚大量暗色颗粒。部分区域可见肾小管变性和坏死。(C) 2002 Elsevier Science Ltd.版权所有。
Histopathological alterations to white seabass, Lates calcar fer aged 3 months in acute and subchronic cadmium exposure were studied by light and scanning electron microscopy. The 96-h LC50 values of cadmium to L. calcarifer was found to be 20.12 +/- 0.61 mg/l and the maximum acceptable toxicant concentration (MATC) was 7.79 mg/l. Fish were exposed to 10 and 0.8 mg/l of Cd (as CdCl2H2O) for 96 h and 90 days, respectively. The study showed that gill lamellae and kidney tubules were the primary target organs for the acute toxic effect of cadmium while in the subchronic exposure, the toxic effect to gills was less than that of kidney and liver. Gill alterations included edema of the epithelial cells with the breakdown of pillar cell system, aneurisms with some ruptures, hypertrophy and hyperplasia of epithelial and chloride cells. The liver showed blood congestion in sinusoids and hydropic swelling of hepatocytes, vacuolation and dark granule accumulation. Lipid droplets and glycogen content were observed in hepatocytes at the second and third month of subchronic exposure. The kidney showed hydropic swelling of tubular cell vacuolation and numerous dark granule accumulation in many tubules. Tubular degeneration and necrosis were seen in some areas. (C) 2002 Elsevier Science Ltd. All rights reserved.