Molecular cloning and characterization of the chicken DNA locus related to the oncogene erbB of avian erythroblastosis virus.
Molecular cloning and characterization of the chicken DNA locus related to the oncogene erbB of avian erythroblastosis virus.
复制标题
与禽成红细胞增多症病毒癌基因 erbB 相关的鸡 DNA 位点的分子克隆和表征。
作者：
A. Sergeant;S. Saule;D. Leprince;A. Begue;C. Rommens;D. Stéhelin
鸡细胞DNA含有与禽成红细胞增多症病毒癌基因v‐erb同源的序列。这些细胞序列（c-erb）是从鸡细胞DNA片段文库中分离出来的，该文库是通过用HaeI和HaeIII部分消化产生的，并且显示出在鸡DNA中至少有两个基因座共享。其中一个被命名为c-erbB，含有大约1.8个与v-erbB癌基因（v-erbB）的3′部分同源的鸡DNA内切酶对。限制性酶切图谱研究表明，与v‐erbB同源的c‐erbB DNA序列分布在位于单个基因组区域的6个EcoRI片段中。病毒RNA中的v-erbB和克隆的c-erbB DNA之间的异源双链体表明，与v-erbB同源的鸡DNA序列被11个不存在于v-erbB癌基因中的DNA序列中断。我们从我们的数据中得出结论，c-erbB基因座可能代表v-erbB癌基因的v-erbB结构域的细胞祖细胞。
Chicken cell DNA contains sequences which are homologous to the avian erythroblastosis virus oncogene v‐erb. These cellular sequences (c‐erb) have been isolated from a library of chicken cell DNA fragments generated by partial digestion with AluI and HaeIII and shown to be shared by at least two loci in the chicken DNA. One of them, denoted c‐erbB, contains approximately 1.8 kilobase pairs of chicken DNA homologous to the 3′ part of the v‐erb oncogene (v‐erbB). Restriction mapping studies show that the c‐erbB DNA sequences homologous to v‐erbB are distributed among six EcoRI fragments located in a single genomic region. Heteroduplexes between v‐erbB in viral RNA and cloned c‐erbB DNA show that the chicken DNA sequences homologous to v‐erbB are interrupted by 11 DNA sequences not present in the v‐erb oncogene. We conclude from our data that the c‐erbB locus might represent the cellular progenitor for the v‐erbB domain of the v‐erb oncogene.