Changing patterns of rotavirus genotypes in Ghana: Emergence of human rotavirus G9 as a major cause of diarrhea in children
Changing patterns of rotavirus genotypes in Ghana: Emergence of human rotavirus G9 as a major cause of diarrhea in children
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DOI：
10.1128/jcm.41.6.2317-2322.2003
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发表时间：
2003-06-01
影响因子：
9.4
通讯作者：
Hall, A
中科院分区：
文献类型：
--
作者：
Armah, GE;Steele, AD;Hall, A
从加纳农村社区上东区的2,205名腹泻幼儿中收集的312份轮状病毒阳性样本进行了人类轮状病毒基因分型。在271株（86.9%）可鉴定为VP 7（G）或VP 4（P）的轮状病毒株中，73株（26.9%）为G9特异性。主要的G9基因型为G9 P [8]，占所有检出G9菌株的79.5%，其次为G9 P [6]（12.3%）、G9 P [10]（2.7%）和G9 P [4]（1.3%）。混合P型的G9菌株占研究中发现的所有G9菌株的2.7%。所有G9 P [8]菌株均具有VP 6亚组II特异性的长RNA电泳图谱。根据粪便材料的丰度选择了4株G9分离株，即GH 1319、GH 1416、GH 3550和GH 3574，它们是观察到的3种电泳型的代表。加纳分离株与来自美国（US 1205）、马拉维（MW 69）、巴西（R160）、日本（95 HI 15）和尼日利亚（Bulumkutu）的其他G9菌株具有超过98%的序列核苷酸同源性。然而，它们与泰国G9株Mc 345仅显示95%的核苷酸同源性。核酸序列的系统发育分析揭示了至少三个簇的存在，加纳菌株形成一个簇，尼日利亚和巴西菌株形成第二个簇，而美国，马拉维和日本菌株形成第三种。
Genotyping of human rotaviruses was performed on 312 rotavirus-positive samples collected from 2,205 young children with diarrhea in the Upper East District of Ghana, a rural community. Of the 271 (86.9%) rotavirus strains that could be VP7 (G) or VP4 (P) characterized, 73 (26.9%) were of G9 specificity. The predominant G9 genotype was G9P[8], which constituted 79.5% of all G9 strains detected, followed by G9P[6] (12.3%), G9P[10] (2.7%), and G9P[4] (1.3%). G9 strains with mixed P types constituted 2.7% of all G9 strains found in the study. All the G9P[8] strains had a long RNA electrophoretic pattern with VP6 subgroup II specificity. Four G9 isolates, GH1319, GH1416, GH3550, and GH3574, which were selected based on the abundance of stool material and were representative of the three electropherotypes observed, were cloned and sequenced. The Ghanaian isolates shared more than 98% sequence nucleotide homology with other G9 strains from the United States (US1205), Malawi (MW69), Brazil (R160), Japan (95HI15), and Nigeria (Bulumkutu). However, they showed only 95% nucleotide homology with the Thai G9 strain Mc345. Phylogenetic analysis of the nucleic acid sequence revealed the existence of at least three clusters, with Ghanaian strains forming one cluster, Nigerian and Brazilian strains forming a second cluster, and U.S., Malawian, and Japanese strains forming a third.