The potential utility of the leopard pleco (Glyptoperichthys gibbiceps) as a biological control of the ciliate protozoan Ichthyophthirius multifiliis
The potential utility of the leopard pleco (Glyptoperichthys gibbiceps) as a biological control of the ciliate protozoan Ichthyophthirius multifiliis
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DOI：
10.1002/ps.2293
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发表时间：
2012-04-01
影响因子：
4.1
通讯作者：
Shinn, Andrew P.
中科院分区：
文献类型：
--
作者：
Picon-Camacho, Sara M.;Leclercq, Eric;Shinn, Andrew P.
背景技术背景：在淡水养殖系统中，纤毛虫原生动物Ichthyophthirius multifiliis Fouquet，1876的感染可导致严重的死亡。在禁止人类食用的鱼类使用孔雀石绿色后，目前可用的化学疗法即使反复使用也仅部分有效。因此，迫切需要确定更有效和更环保的控制战略。本研究采用一种以金钱豹为食藻基质的生物制剂，对金钱豹的生长发育、繁殖、繁殖和生长状况进行了研究。多丝状体感染的蓝罗非鱼，奥利亚罗非鱼，在温水中饲养，实验坦克为基础的系统进行了探索。multifiliis对生物膜覆盖的基质表现出选择性沉降行为。在受控暴露后，I. multifiliis trophons在蓝罗非鱼上的建立，O.金黄色葡萄球菌（G. aureus），为4.9 ± 0.4;结果表明，在实验条件下，猪的生长速度明显低于单独饲养的猪（13.3 ± 0.9; ANOVA，P <0.001）。赤肱二头肌作为生物防治降低I. multifiliis对暖水系统中具有商业价值的鱼类种群的影响。这可能与G.在生物膜层上的gibbiceps，I. multifiliis显示优先沉降。针对病原性原生动物种类如I.如果在适当的情况下结合针对寄生虫外部繁殖生命阶段的生物控制措施，可能会大大减少目前对化学干预措施的依赖，从而使多丝寄生虫受益。(C)2012年化学工业协会
BACKGROUND: Infections of the ciliate protozoan Ichthyophthirius multifiliis Fouquet, 1876, can cause heavy mortalities in freshwater aquaculture systems. Following the ban of malachite green on fish for human consumption, currently available chemical therapies are only partially efficacious even when repeatedly applied. There is, therefore, an urgent necessity to identify more efficient and environmentally friendly control strategies. In the present study, the use of a substrate algae feeder leopard pleco, Glyptoperichthys gibbiceps, as a biological agent to control I. multifiliis infections on blue tilapia, Oreochromis aureus, reared in a warm-water, experimental tank-based system is explored.RESULTS: The free-swimming protomont stage of I. multifiliis demonstrated selective settlement behaviour towards biofilmcovered substrates. Following a controlled exposure, the number of I. multifiliis trophonts establishing on naive blue tilapia, O. aureus, was 4.9 +/- 0.4 when reared with G. gibbiceps, significantly lower than when reared in isolation ( 13.3 +/- 0.9; ANOVA, P < 0.001).CONCLUSION: This study demonstrates for the first time the potential of G. gibbiceps as a biological control to reduce I. multifiliis on commercially valuable fish stocks in warm-water systems. This likely originated from the grazing activity of G. gibbiceps on the biofilm layer upon which the multiplicative stage of I. multifiliis was shown preferentially to settle. Therapeutic strategies against pathogenic protozoan species like I. multifiliis could greatly benefit from incorporating, where appropriate, a biological control targeting the external multiplicative life stagesof the parasite, potentially reducing the current dependence onchemical interventions. (C) 2012 Society of Chemical Industry