Analysis of cellular senescence in culture in vivo: the senescence-associated beta-galactosidase assay.
Analysis of cellular senescence in culture in vivo: the senescence-associated beta-galactosidase assay.
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DOI：
10.1002/0471143030.cb1809s27
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发表时间：
2005-07-01
影响因子：
--
通讯作者：
Medrano, Estela E
中科院分区：
文献类型：
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作者：
Bandyopadhyay, Debdutta;Gatza, Catherine;Medrano, Estela E
复制性衰老是一个大约 40 年前首次被描述的过程，它会导致不可逆的生长停滞和持续的代谢功能，并且还与细胞凋亡信号的抵抗力增强有关。过去十年来，人们对这一过程的兴趣大大增加，因为事实证明，衰老可以作为一种有效的肿瘤抑制机制。尽管越来越多的证据表明衰老表型与一系列异常复杂的基因表达模式以及与微环境的相互作用有关，但只有一种广泛接受的标记可以在体外和体内区分此类细胞。该标记是 pH 6 β-半乳糖苷酶 (SA-β-gal) 的衰老相关表达。本文描述了一种分析培养细胞以及人类和动物组织中 SA-β-gal 表达的方法，并强调了进行此类测定时需要考虑的重要参数。
Replicative senescence, a process first described almost 40 years ago, entails irreversible growth arrest with sustained metabolic functions, and it is also associated with increased resistance to apoptotic signals. Interest in this process has increased greatly over the last 10 years, as it has been demonstrated that senescence can function as a potent tumor suppressor mechanism. Although mounting evidence suggests that the senescent phenotype is associated with an extraordinarily complex array of gene expression patterns and interactions with the microenvironment, there is only one widely accepted marker for distinguishing such cells in vitro and in vivo. This marker is the senescence-associated expression of a pH 6 beta-galactosidase (SA-beta-gal). Here, a method for analyzing SA-beta-gal expression in cultured cells and in human and animal tissues is described, and important parameters to consider when performing such assays are also highlighted.