Gene Replacement and Transposon Delivery Using the Negative Selection Marker sacB.
Gene Replacement and Transposon Delivery Using the Negative Selection Marker sacB.
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DOI：
10.1385/1-59259-147-7:059
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发表时间：
2001-01-01
期刊：
影响因子：
--
通讯作者：
Guilhot, C
中科院分区：
文献类型：
--
作者：
Jackson, M;Reinaldo Camacho, L;Guilhot, C
基因置换和转座子诱变是两种互补的工具，已被广泛用于在各种生物体中进行遗传研究。在分枝杆菌中，特别是在结核分枝杆菌复合体中，这些工具的缺乏严重阻碍了这些生物体的遗传研究，特别是关于这些重要病原体的毒力基因的鉴定和表征。
Gene replacement and transposon mutagenesis are two complementary tools that have been widely used to perform genetic studies in various living organisms. In mycobacteria, and especially in the Mycobacterium tuberculosis complex, the lack of these tools has severely hampered the genetic studies of these organisms, especially regarding the identification and characterization of virulence genes of these important pathogens.