Effect of atriopeptin II on determinants of glomerular filtration rate in the in vitro perfused dog glomerulus.
Effect of atriopeptin II on determinants of glomerular filtration rate in the in vitro perfused dog glomerulus.
复制标题
心肽素 II 对体外灌注狗肾小球肾小球滤过率决定因素的影响。
DOI：
10.1152/ajprenal.1986.250.6.f1119
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Stein,JH
中科院分区：
文献类型：
--
作者：
Fried,TA;McCoy,RN;Osgood,RW;Stein,JH
心房利钠因子（ANF）是一种最初发现存在于哺乳动物心房提取物中的肽，具有显著的利钠和利尿性质。人们提出了许多机制来解释这些特性。最近，有研究表明ANF可能增强肾小球滤过。在这份报告中，我们描述了一系列的实验，旨在研究心房肽素II，一个23个氨基酸的合成类似物的心钠素，增加肾小球滤过率（GFR），如果是这样，这种增加的机制。我们使用了孤立的灌注肾小球技术（n = 6），它允许一个单一的孤立的肾小球单位进行灌注和单肾单位GFR（SNGFR）的决定因素进行测量。每个实验进行两个阶段，控制，然后是实验。两个阶段之间的唯一区别是在实验灌注液中加入终浓度为5 × 10（-7）M的心房肽素II。SNGFR确实显著增加（78 +/- 27至108 +/- 29 nl/min，P <0.01）。这种增加与肾小球毛细血管静水压力（PGC）从31 +/- 3 mmHg显著增加至35 +/- 3 mmHg相关（P <0.05）。在每个实验中，滤过分数也增加（从0.16 +/- 0.3增加到0.25 +/- 0.03，P小于0.005）。无论是传入流量，也不传出小动脉流量的变化，虽然有一个趋势，两者都减少。（250字处删节）
Atrial natriuretic factor (ANF) is a peptide originally found to be present in extracts of mammalian atria that possess marked natriuretic and diuretic qualities. A number of mechanisms have been suggested to explain these properties. Recently, it has been suggested that ANF may enhance glomerular filtration. In this report, we describe a series of experiments designed to investigate if atriopeptin II, a 23-amino acid synthetic analogue of ANF, increases glomerular filtration rate (GFR) and, if so, the mechanism for this increase. We used the isolated perfused glomerulus technique (n = 6), which allows a single isolated glomerular unit to be perfused and the determinants of single-nephron GFR (SNGFR) to be measured. Two periods were performed in each experiment, the control followed by the experimental. The only difference between the two periods was the addition of atriopeptin II to the experimental perfusate at a final concentration of 5 X 10(-7) M. There was indeed a significant increase in the SNGFR (78 +/- 27 to 108 +/- 29 nl/min, P less than 0.01). This increase was associated with a significant increase in the glomerular capillary hydrostatic pressure (PGC) from 31 +/- 3 to 35 +/- 3 mmHg (P less than 0.05). The filtration fraction also increased in each experiment (from 0.16 +/- 0.3 to 0.25 +/- 0.03, P less than 0.005). Neither the afferent flow nor the efferent arteriolar flow changed, although there was a tendency for both to decrease.(ABSTRACT TRUNCATED AT 250 WORDS)