Evaluation of the reliability of chromosomal imbalances detected by combined use of universal DNA amplification and comparative genomic hybridization.
Evaluation of the reliability of chromosomal imbalances detected by combined use of universal DNA amplification and comparative genomic hybridization.
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DOI：
10.1111/j.1349-7006.2000.tb00894.x
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发表时间：
2000-11
期刊：
影响因子：
--
通讯作者：
Sasaki K
中科院分区：
文献类型：
--
作者：
Harada T;Shiraishi K;Kusano N;Umayahara K;Kondoh S;Okita K;Sasaki K
比较基因组杂交(CGH)分析显微肿瘤样本是通过使用简并寡核苷酸引物聚合酶链式反应(DOP-PCR)的通用DNA扩增而实现的。为了评价DOP-PCRCGH的可靠性，我们对10例肝胆管和胰腺恶性肿瘤的DNA进行了DOP-PCRCGH和标准CGH的平行检测。两种方法获得的结果相似，但有少数例外，表明DOP-PCR CGH提供了与标准CGH相同的细胞遗传学信息。我们还研究了DOP-PCRCGH的敏感性，使用的是从显微解剖的肿瘤细胞中提取的DNA的顺序稀释。使用100~800 pg的模板DNA进行DOP-PCR可获得成功的CGH结果。但模板DNA的产生量较少，不宜超过50pg。这些结果表明DOP-PCR计算全息适用于对标准计算全息来说太小的微小样品的计算全息分析。因此，DOP-PCRCGH分析有可能成为临床实验室检测的有用方法。
Comparative genomic hybridization (CGH) analysis of microscopic tumor samples is allowed by universal DNA amplification using degenerate oligonucleotide primed‐PCR (DOP‐PCR). To evaluate the reliablity of DOP‐PCR CGH, we performed DOP‐PCR CGH and standard CGH in parallel using DNAs extracted from 10 malignant tumors of the hepatobiliary tract and pancreas. Similar results were obtained by both methods with a few exceptions, indicating that DOP‐PCR CGH provides cytogenetic information equivalent to that obtained from standard CGH. We also investigated the sensitivity of DOP‐PCR CGH using sequential dilutions of DNA from microdissected tumor cells. DOP‐PCR using 100 to 800 pg of template DNA yielded successful CGH results. However, less than 50 pg of template DNA was not suitable because of the small amount of generated DNA. These findings suggest that DOP‐PCR CGH is applicable for CGH analysis of tiny specimens which are too small for standard CGH. Accordingly, DOP‐PCR CGH analysis may become a useful method in clinical laboratory examination.
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影响因子：
--
作者：
Aubele, M;Mattis, A;Werner, M
通讯作者：
Werner, M
影响因子：
2.7
作者：
Sanchez-Cespedes, M;Cairns, P;Sidransky, D
通讯作者：
Sidransky, D
影响因子：
29.4
作者：
Willenbucher, RF;Zelman, SJ;Waldman, FM
通讯作者：
Waldman, FM
DOI：
10.1073/pnas.93.25.14676
发表时间：
1996-12-10
影响因子：
11.1
作者：
Cheung, VG;Nelson, SF
通讯作者：
Nelson, SF
影响因子：
4.4
作者：
TELENIUS, H;CARTER, NP;TUNNACLIFFE, A
通讯作者：
TUNNACLIFFE, A