A low cost point-of-care viscous sample preparation device for molecular diagnosis in the developing world; an example of microfluidic origami
A low cost point-of-care viscous sample preparation device for molecular diagnosis in the developing world; an example of microfluidic origami
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DOI：
10.1039/c1lc20622b
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发表时间：
2012-01-01
期刊：
影响因子：
6.1
通讯作者：
Boehringer, K. F.
中科院分区：
文献类型：
--
作者：
Govindarajan, A. V.;Ramachandran, S.;Boehringer, K. F.
芯片实验室的概念已经导致了几个护理点(POC)诊断微流控平台。然而，这些设备中几乎没有几个可以处理用于分子诊断的原始样本，更少的设备适合在没有永久电气基础设施的资源有限的环境中使用。我们在这里提出了一种非常低成本的纸质微流控设备，用于从原始粘性样本中提取细菌DNA-这是传统微流控平台面临的挑战。这是一个“微流控折纸”的例子，通过折叠激活系统；这里展示了室温细胞裂解和从添加了大肠杆菌的猪粘蛋白(模拟痰)中提取DNA，而不需要使用外部电源。微流体折纸设备的特点是干燥的试剂存储和裂解缓冲液的再水化。我们演示了从细菌负载低至33CFU毫升(-1)的样本中提取DNA。从原始样品开始，提取时间已优化到约1.5小时，而不使用外部电源，或者使用烤箱或加热器块在1小时内。这种纸质微流控器件的制造可以转化为发展中国家的大批量生产，而不需要半导体洁净室或微加工设施。只需向装置中添加样品、水、乙醇和洗脱缓冲液即可进行样品制备，从而减少运输和处理过程中的化学危害。
The lab-on-a-chip concept has led to several point-of-care (POC) diagnostic microfluidic platforms. However, few of these can process raw samples for molecular diagnosis and fewer yet are suited for use in a resource-limited setting without permanent electrical infrastructure. We present here a very low cost paper microfluidic device for POC extraction of bacterial DNA from raw viscous samples-a challenge for conventional microfluidic platforms. This is an example of "microfluidic origami" in that the system is activated by folding; demonstrated here is room temperature cell lysis and DNA extraction from pig mucin (simulating sputum) spiked with E. coli without the use of external power. The microfluidic origami device features dry reagent storage and rehydration of the lysis buffer. We demonstrate DNA extraction from samples with a bacterial load as low as 33 CFU ml(-1). Extraction times, starting from the raw sample, have been optimized to about 1.5 h without the use of external power, or to within 1 h using an oven or a heater block. The fabrication of this paper microfluidic device can be translated into high volume production in the developing world without the need for a semiconductor clean room or a microfabrication facility. The sample preparation can be performed with the addition of just the sample, water, ethanol and elute buffer to the device, thus reducing chemical hazards during transport and handling.