Sudan Black B, The Specific Histochemical Stain for Lipofuscin: A Novel Method to Detect Senescent Cells
Sudan Black B, The Specific Histochemical Stain for Lipofuscin: A Novel Method to Detect Senescent Cells
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DOI：
10.1007/978-1-4939-6670-7_10
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发表时间：
2017-01-01
期刊：
影响因子：
--
通讯作者：
Gorgoulis, Vassilis G.
中科院分区：
文献类型：
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作者：
Evangelou, Konstantinos;Gorgoulis, Vassilis G.
众所周知，苏丹黑B(SBB)组织化学染色对脂褐素有特异性反应，脂褐素是一种氧化蛋白质、脂类和金属的聚集体。脂褐素与许多衰老过程有关。众所周知，它也会在衰老的细胞中积累。我们最近证明，当应用SBB染色时，脂褐素检测对于衰老细胞的可视化具有高度的特异性。在这里，我们详细介绍了这种SBB方法，它可以检测任何材料中的衰老细胞，而无论其制备方式如何。这不仅在理解各种疾病的生理过程和病理生理学方面提供了独特的优势，而且在估计对治疗干预的反应方面也提供了独特的优势。
The Sudan-Black-B (SBB) histochemical stain is well known to specifically react against lipofuscin, an aggregate of oxidized proteins, lipids, and metals. Lipofuscin is related to many ageing processes. It is also known to accumulate in senescent cells. We recently proved that lipofuscin detection, when applying the SBB staining, is highly specific for the visualization of senescent cells. Here, we present in detail this SBB method that can detect senescent cells in any material, irrespective of its preparation. This provides unique advantages not only in understanding physiological processes and the pathophysiology of various diseases but also in estimating the response to therapeutic interventions.