Peripheral frequency of CD4+ CD28- cells in acute ischemic stroke: relationship with stroke subtype and severity markers.
Peripheral frequency of CD4+ CD28- cells in acute ischemic stroke: relationship with stroke subtype and severity markers.
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DOI：
10.1097/md.0000000000000813
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发表时间：
2015-05
期刊：
影响因子：
1.6
通讯作者：
Pinto A
中科院分区：
文献类型：
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作者：
Tuttolomondo A;Pecoraro R;Casuccio A;Di Raimondo D;Buttà C;Clemente G;Corte VD;Guggino G;Arnao V;Maida C;Simonetta I;Maugeri R;Squatrito R;Pinto A
据报道，CD4+ CD28− T细胞也称为CD28 null细胞，在急性冠状动脉综合征的临床环境中增加。之前只有2项研究分析了急性缺血性卒中受试者中CD28无效细胞的外周频率，但据我们所知，从未评价过缺血性卒中各吐司亚型中CD28无效细胞的外周频率。我们假设CD4+细胞，特别是CD28空细胞亚群在急性缺血性卒中受试者中与缺血性卒中的临床亚型和严重程度相关，可以显示出不同程度的外周血百分比。我们研究的目的是分析急性缺血性中风受试者中CD 28空细胞的外周频率与吐司诊断亚型的关系，并评估它们与急性缺血性中风临床严重程度评分的关系，以及它们在诊断中的预测作用急性缺血性中风和诊断亚型我们招募了98名诊断为缺血性中风的连续受试者。作为对照，我们招募了66名未诊断出急性缺血性中风的住院患者。用FACS Calibur流式细胞仪评价了CD4+和CD28无效细胞的外周频率。急性缺血性卒中受试者外周血CD4+细胞和CD28空细胞的频率显著高于无急性缺血性卒中的对照受试者。与其他吐司亚型受试者相比，心源性栓塞性卒中受试者的外周血CD 4+细胞和CD 28空细胞频率显著更高。我们观察到外周血CD28空细胞百分比与斯堪的纳维亚卒中量表和NIHSS评分之间存在显著相关性。ROC曲线分析表明，CD28空细胞百分比可能有助于区分卒中亚型。这些发现似乎表明T细胞成分在急性缺血性中风临床环境中也可能起作用，显示与中风的每种吐司亚型相关的不同外周频率的CD28无效细胞。
CD4+ CD28− T cells also called CD28 null cells have been reported as increased in the clinical setting of acute coronary syndrome. Only 2 studies previously analyzed peripheral frequency of CD28 null cells in subjects with acute ischemic stroke but, to our knowledge, peripheral frequency of CD28 null cells in each TOAST subtype of ischemic stroke has never been evaluated. We hypothesized that CD4+ cells and, in particular, the CD28 null cell subset could show a different degree of peripheral percentage in subjects with acute ischemic stroke in relation to clinical subtype and severity of ischemic stroke. The aim of our study was to analyze peripheral frequency of CD28 null cells in subjects with acute ischemic stroke in relation to TOAST diagnostic subtype, and to evaluate their relationship with scores of clinical severity of acute ischemic stroke, and their predictive role in the diagnosis of acute ischemic stroke and diagnostic subtype We enrolled 98 consecutive subjects admitted to our recruitment wards with a diagnosis of ischemic stroke. As controls we enrolled 66 hospitalized patients without a diagnosis of acute ischemic stroke. Peripheral frequency of CD4+ and CD28 null cells has been evaluated with a FACS Calibur flow cytometer. Subjects with acute ischemic stroke had a significantly higher peripheral frequency of CD4+ cells and CD28 null cells compared to control subjects without acute ischemic stroke. Subjects with cardioembolic stroke had a significantly higher peripheral frequency of CD4+ cells and CD28 null cells compared to subjects with other TOAST subtypes. We observed a significant relationship between CD28 null cells peripheral percentage and Scandinavian Stroke Scale and NIHSS scores. ROC curve analysis showed that CD28 null cell percentage may be useful to differentiate between stroke subtypes. These findings seem suggest a possible role for a T-cell component also in acute ischemic stroke clinical setting showing a different peripheral frequency of CD28 null cells in relation of each TOAST subtype of stroke.