Shaping axial identity during human pluripotent stem cell differentiation to neural crest cells.
Shaping axial identity during human pluripotent stem cell differentiation to neural crest cells.
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DOI：
10.1042/bst20211152
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发表时间：
2022-02-28
影响因子：
3.9
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中科院分区：
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神经嵴（NC）是多能细胞群，其可产生大量衍生物，包括周围神经系统的神经元和神经胶质、软骨、心脏平滑肌、黑素细胞和交感肾上腺细胞。模拟人NC发育和相关出生缺陷以及产生用于再生医学应用的临床相关细胞群的有吸引力的策略涉及从人多能干细胞（hPSC）体外产生NC。然而，在体内，NC细胞产生不同细胞类型的潜力由它们沿前后（A-P）轴的沿着位置决定，因此hPSC衍生的NC细胞的轴向身份是要考虑的重要方面。最近的进展，了解NC的发育起源和参与其规范的信号转导途径，有助于在体外产生的人NC细胞是代表各种A-P位置。在这里，我们探讨了最近的进展，在体外NC规格和轴图案化使用hPSC的方法。
The neural crest (NC) is a multipotent cell population which can give rise to a vast array of derivatives including neurons and glia of the peripheral nervous system, cartilage, cardiac smooth muscle, melanocytes and sympathoadrenal cells. An attractive strategy to model human NC development and associated birth defects as well as produce clinically relevant cell populations for regenerative medicine applications involves the in vitro generation of NC from human pluripotent stem cells (hPSCs). However, in vivo, the potential of NC cells to generate distinct cell types is determined by their position along the anteroposterior (A–P) axis and, therefore the axial identity of hPSC-derived NC cells is an important aspect to consider. Recent advances in understanding the developmental origins of NC and the signalling pathways involved in its specification have aided the in vitro generation of human NC cells which are representative of various A–P positions. Here, we explore recent advances in methodologies of in vitro NC specification and axis patterning using hPSCs.