Differential detection of transmissible gastroenteritis virus and porcine epidemic diarrhea virus by duplex RT-PCR
Differential detection of transmissible gastroenteritis virus and porcine epidemic diarrhea virus by duplex RT-PCR
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DOI：
10.1177/104063870101300611
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发表时间：
2001-11-01
影响因子：
1.5
通讯作者：
Park, BK
中科院分区：
文献类型：
--
作者：
Kim, SY;Song, DS;Park, BK
传染性胃肠炎（TGE）和猪流行性腹泻（PED）是仔猪高度传染性的肠道疾病。这些疾病的临床症状非常相似，包括淡黄色水样腹泻。因此，需要对TGE病毒和PED病毒进行有效的鉴别检测。本研究建立了一种双链逆转录聚合酶链反应（RT-PCR）方法，用于区分TGE病毒和PED病毒。引物分别针对每种病毒的S基因设计。从腹泻猪的肠道和粪便样本中提取RNA。RT-PCR检测结果显示，2 TCID50/200 μ l均能检测到TGE病毒和PED病毒。在90份临床样本中，双相RT-PCR检测到7种TGE病毒和2种PED病毒。该双相RT-PCR方法可作为一种快速、特异、灵敏的TGE和PED病毒鉴别检测方法。
Transmissible gastroenteritis (TGE) and porcine epidemic diarrhea (PED) are highly contagious enteric diseases of piglets. The clinical signs of these diseases are very similar and include watery, yellowish diarrhea. Thus, the effective differential detection of TGE virus and PED virus is required. In the present study, a duplex reverse transcription-polymerase chain reaction (RT-PCR) was established for the differential detection of TGE and PED viruses. The primers were designed for the S gene of each virus. RNA was extracted from the intestines and stool samples that were collected from the swine with diarrhea. The RT-PCR test could detect both TGE and PED viruses with 2 TCID50/200 mul. Among 90 clinical samples, 7 TGE viruses and 2 PED viruses were detected by the duplex RT-PCR. This duplex RT-PCR may be a useful diagnostic method for the rapid, specific, and sensitive differential detection of TGE and PED viruses using clinical samples.