Studies of the transferability of microsatellites derived from Triticum tauschii to hexaploid wheat and to diploid related species using amplification, hybridization and sequence comparisons
Studies of the transferability of microsatellites derived from Triticum tauschii to hexaploid wheat and to diploid related species using amplification, hybridization and sequence comparisons
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DOI：
10.1007/s00122-002-0963-8
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发表时间：
2002-06
影响因子：
5.4
通讯作者：
H. Guyomarc'h;P. Sourdille;Keith J. Edwards;M. Bernard
中科院分区：
文献类型：
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作者：
H. Guyomarc'h;P. Sourdille;Keith J. Edwards;M. Bernard
六倍体小麦 (Triticum aestivumL em Thell) 源自复杂的杂交程序，涉及三个分别携带 A、B 和 D 基因组的二倍体物种。我们最近从 aT 中分离出了微卫星。 tauschiilibrary 富集了各种基序，并评估了这些标记向携带 A、B 或 D 基因组的几个二倍体物种的可转移性。所有引物对都扩增面包小麦上的一个以上基因座，而提供 D 基因组特异性基因座的引物对的一半则在 A 和/或 B 二倍体物种上产生扩增产物。所有标记均给出 T 的单一扩增产物。 tauschii 和其他二倍体物种上没有扩增在六倍体水平上具有 D 基因组特异性。非特异性微卫星标记（在携带 A、B 或 D 基因组的二倍体物种上产生扩增产物）在面包小麦上产生复杂的扩增模式（具有多个条带）或生成映射到 D 基因组的单个条带。用对应于微卫星侧翼区域的探针进行的 Southern 印迹杂交在所有二倍体和六倍体物种上都给出了信号，无论微卫星的特异性如何。在面包小麦上观察到的模式通常与在二倍体物种中观察到的模式一致，只是有轻微的重排。这表明微卫星标记的特异性可能是由于微卫星侧翼区域的突变而不是多倍化事件期间的序列消除，并且多倍体水平的基因组严格性高于二倍体水平。
Hexaploid wheat (Triticum aestivumL em Thell) is derived from a complex hybridization procedure involving three diploid species carrying the A, B and D genomes, respectively. We recently isolated microsatellites from aT. tauschiilibrary enriched for various motifs and evaluated the transferability of these markers to several diploid species carrying the A, B or D genomes. All of the primer pairs amplifying more than one locus on bread wheat and half of those giving D-genome-specific loci gave an amplification product on A-and/or B-diploid species. All of the markers giving a single amplification product forT. tauschiiand no amplification on the other diploid species were D-genome-specific at the hexaploid level. The non-specific microsatellite markers (which gave an amplification product on diploid species carrying the A, B or D genome) gave either a complex amplification pattern on bread wheat (with several bands) or generated a single band which mapped to the D genome. Southern blot hybridizations with probes corresponding to the microsatellite flanking regions gave a signal on all diploid and hexaploid species, whatever the specificity of the microsatellite. The patterns observed on bread wheat were generally in accordance with those observed for diploid species, with slight rearrangements. This suggests that the specificity of microsatellite markers is probably due to mutations in microsatellite flanking regions rather than sequence elimination during polyploidization events and that genome stringency is higher at the polyploid than at the diploid level.