Role of Candida species from HIV infected children in enamel caries lesions: an in vitro study.
Role of Candida species from HIV infected children in enamel caries lesions: an in vitro study.
复制标题
DOI：
10.1590/1678-77572016-0021
复制
发表时间：
2017-01
期刊：
影响因子：
--
通讯作者：
Castro GF
中科院分区：
文献类型：
--
作者：
Charone S;Portela MB;Martins KO;Soares RM;Castro GF
本研究对假丝酵母菌的产生量进行了分析。用横向显微硬度计(TMH)、偏光显微镜(PLM)和牙釉质钙离子释放量(Ca~(2+))对HIV感染(HIV+)儿童牙本质生物膜进行体外脱矿。假丝酵母菌样本从HIV阳性儿童的牙龈上生物膜中分离。将140个釉质块随机分为6组：白色念珠菌生物被膜组(第1组)、白色念珠菌与热带念珠菌混合生物膜组(第2组)、白色念珠菌与近缘念珠菌混合生物膜组(第3组)、白色念珠菌、近缘念珠菌与光滑念珠菌混合生物膜组(第4组)、白色念珠菌ATCC生物被膜组(第5组)、无念珠菌混合生物膜组(第6组)。分别于生物膜形成后第3、5、8、15天取出釉质块评价TMH，并用激光共聚焦显微镜对釉质图像进行分析。用原子吸收光谱仪测定第1组和第6组的钙离子释放量。统计分析采用SPSS统计软件，显著性水平为5%。TMH组牙釉质硬度在第1~15天逐渐降低(p<；0.05)，但主要集中在生物膜形成后的第5天。PLM显示浅表病变，显示孔隙率增加。在生物膜形成过程中，白色念珠菌引起悬浮液中钙离子的释放。HIV阳性儿童牙科生物膜中的念珠菌在体外可引起乳牙釉质脱矿。
This study analyzed the capacity of Candida spp. from dental biofilm of HIV infected (HIV+) children to demineralize primary molar enamel in vitro by Transversal Microhardness (TMH), Polarized Light Microscopy (PLM) and the quantity of calcium ions (Ca2+) released from the enamel. Candida spp. samples were isolated from the supragingival biofilm of HIV+ children. A hundred and forty (140) enamel blocks were randomly assigned to six groups: biofilm formed by C. albicans (Group 1); mixed biofilm formed by C. albicans and C. tropicalis (Group 2); mixed biofilm formed by C. albicans and C. parapsilosis (Group 3); mixed biofilm formed by C. albicans, C. parapsilosis and C. glabrata (Group 4); biofilm formed by C. albicans ATCC (Group 5) and medium without Candida (Group 6). Enamel blocks from each group were removed on days 3, 5, 8 and 15 after biofilm formation to evaluate the TMH and images of enamel were analyzed by PLM. The quantity of Ca2+ released, from Groups 1 and 6, was determined using an Atomic Absorption Spectrophotometer. The SPSS program was used for statistical analysis and the significance level was 5%. TMH showed a gradual reduction in enamel hardness (p<0.05) from the 1st to 15th day, but mainly five days after biofilm formation in all groups. The PLM showed superficial lesions indicating an increase in porosity. C. albicans caused the release of Ca2+ into suspension during biofilm formation. Candida species from dental biofilm of HIV+ children can cause demineralization of primary enamel in vitro.