Differential macrophage polarization promotes tissue remodeling and repair in a model of ischemic retinopathy.
Differential macrophage polarization promotes tissue remodeling and repair in a model of ischemic retinopathy.
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DOI：
10.1038/srep00076
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发表时间：
2011
影响因子：
4.6
通讯作者：
Friedlander, Martin
中科院分区：
文献类型：
--
作者：
Marchetti, Valentina;Yanes, Oscar;Aguilar, Edith;Wang, Matthew;Friedlander, David;Moreno, Stacey;Storm, Kathleen;Zhan, Min;Naccache, Samia;Nemerow, Glen;Siuzdak, Gary;Friedlander, Martin
糖尿病视网膜病变是55岁以下人群视力丧失的主要原因。脐带血（UCB）衍生的髓样祖细胞已被证明可以减少中枢神经系统中与缺血相关的神经元损伤。在这项研究中，我们发现脐血来源的CD14+祖细胞通过促进生理性血管生成和减少相关炎症在小鼠缺血性视网膜病变模型中提供了拯救作用。我们使用共聚焦显微镜追踪注射的人脐血来源的CD14+细胞的命运，并使用种特异性探针进行PCR，以研究注射前后的基因表达谱。代谢组学分析测量由CD14+细胞诱导的变化。我们的研究结果表明，人类细胞在体内分化成M2巨噬细胞，并诱导居民M2巨噬细胞的极化。这通过调节炎症反应、减少氧化应激和细胞凋亡以及促进组织修复来稳定缺血损伤的视网膜脉管系统。
Diabetic retinopathy is the leading cause of visual loss in individuals under the age of 55. Umbilical cord blood (UCB)–derived myeloid progenitor cells have been shown to decrease neuronal damage associated with ischemia in the central nervous system. In this study we show that UCB-derived CD14+ progenitor cells provide rescue effects in a mouse model of ischemic retinopathy by promoting physiological angiogenesis and reducing associated inflammation. We use confocal microscopy to trace the fate of injected human UCB-derived CD14+ cells and PCR with species-specific probes to investigate their gene expression profile before and after injection. Metabolomic analysis measures changes induced by CD14+ cells. Our results demonstrate that human cells differentiate in vivo into M2 macrophages and induce the polarization of resident M2 macrophages. This leads to stabilization of the ischemia-injured retinal vasculature by modulating the inflammatory response, reducing oxidative stress and apoptosis and promoting tissue repair.
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影响因子：
12.4
作者：
Nepomuceno, Ronald R.;Pache, Lars;Nemerow, Glen R.
通讯作者：
Nemerow, Glen R.
影响因子：
20.3
作者：
Fantin, Alessandro;Vieira, Joaquim M.;Ruhrberg, Christiana
通讯作者：
Ruhrberg, Christiana
影响因子：
5.2
作者：
Kuwana, Masataka;Okazaki, Yuka;Ikeda, Yasuo
通讯作者：
Ikeda, Yasuo
影响因子：
4
作者：
Kodama, H;Inoue, T;Kuwana, M
通讯作者：
Kuwana, M
影响因子：
3.7
作者：
Gustafsson C;Mjösberg J;Matussek A;Geffers R;Matthiesen L;Berg G;Sharma S;Buer J;Ernerudh J
通讯作者：
Ernerudh J