Enhanced Stability of DNA Nanostructures by Incorporation of Unnatural Base Pairs
Enhanced Stability of DNA Nanostructures by Incorporation of Unnatural Base Pairs
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通过掺入非天然碱基对 (UBP) 增强 DNA 纳米结构的稳定性
DOI：
10.1002/cphc.201700809
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发表时间：
2017-11-03
期刊：
影响因子：
2.9
通讯作者：
Chen, Fei
中科院分区：
文献类型：
--
作者：
Liu, Qing;Liu, Guocheng;Chen, Fei
自组装DNA纳米结构在纳米制造、生物传感和纳米医学等领域具有广阔的应用前景。然而，DNA双螺旋结构固有的低稳定性是由弱相互作用形成的，在很大程度上阻碍了DNA纳米结构的组装和功能。在这项研究中，我们通过将非自然碱基对5-Me-isoC/isoG和a /2-thioT结合到双螺旋中，重新设计并构建了一个六臂DNA连接。它们不仅保持了DNA纳米结构的完整性，而且表现出增强的热稳定性和对T7外切酶酶切的抗性。该研究将进一步拓展DNA纳米结构在纳米制造和生物医学领域的应用，并且利用非自然碱基对进行基因序列扩展，可以构建更复杂、更多样化的自组装DNA纳米结构。
Self-assembled DNA nanostructures hold great promise in the fields of nanofabrication, biosensing and nanomedicine. However, the inherent low stability of the DNA double helices, formed by weak interactions, largely hinders the assembly and functions of DNA nanostructures. In this study, we redesigned and constructed a six-arm DNA junction by incorporation of the unnatural base pairs 5-Me-isoC/isoG and A/2-thioT into the double helices. They not only retained the structural integrity of the DNA nanostructure, but also showed enhanced thermal stability and resistance to T7 Exonuclease digestion. This research may expand the applications of DNA nanostructures in nanofabrication and biomedical fields, and furthermore, the genetic alphabet expansion with unnatural base pairs may enable us to construct more complicated and diversified self-assembled DNA nanostructures.