Biofilm formation in medicated root canals
Biofilm formation in medicated root canals
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DOI：
10.1097/00004770-200210000-00003
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发表时间：
2002-10-01
影响因子：
4.2
通讯作者：
Gillespie, MJ
中科院分区：
文献类型：
--
作者：
Distel, JW;Hatton, JF;Gillespie, MJ
粪肠球菌通过形成生物膜抵抗常见根管内药物的假设得到了验证。E.用扫描电镜和激光共聚焦显微镜观察了46个中药根的粪菌定植情况。扫描电镜观察到氢氧化钙尖和阳性对照组根管在2天内定植。扫描电镜检测到生物膜的根管与氢氧化钙糊剂平均77天。扫描共聚焦激光显微镜分析两个氢氧化钙糊剂药物根显示可行的菌落形成在根管感染86天，而在一个管感染160天，蘑菇形典型的生物膜obliferations.Analysis十二烷基硫酸钠聚丙烯酰胺凝胶电泳分析表明，在自由浮动（反渗透）和接种培养物的细菌蛋白质谱之间没有差异。生物膜细菌的分析是不确定的。药物根管内粪生物膜的形成。
The hypothesis that Enterococcus faecalis resists common intracanal medications by forming biofilms was tested. E. faecalis colonization of 46 extracted, medicated roots was observed with scanning electron microscopy (SEM) and scanning confocal laser microscopy. SEM detected colonization of root canals medicated with calcium hydroxide points and the positive control within 2 days. SEM detected biofilms in canals medicated with calcium hydroxide paste in an average of 77 days. Scanning confocal laser microscopy analysis of two calcium hydroxide paste medicated roots showed viable colonies forming in a root canal infected for 86 days, whereas in a canal infected for 160 days, a mushroom-shape typical of a biofilm was observed.Analysis by sodium dodecyl sulfate polyacrylamide gel electrophoresis showed no differences between the protein profiles of bacteria in free-floating (planktonic) and inoculum cultures. Analysis of biofilm bacteria was inconclusive.These observations support potential E. faecalis biofilm formation in vivo in medicated root canals.