The OPAQUE1/DISCORDIA2 myosin XI is required for phragmoplast guidance during asymmetric cell division in maize
The OPAQUE1/DISCORDIA2 myosin XI is required for phragmoplast guidance during asymmetric cell division in maize
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DOI：
10.1093/plcell/koad099
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发表时间：
2023-04-05
期刊：
影响因子：
11.6
通讯作者：
Facette, Michelle R.
中科院分区：
文献类型：
--
作者：
Nan, Qiong;Liang, Hong;Facette, Michelle R.
形成性不对称分裂产生不同命运的细胞，对发育至关重要。我们发现玉米（Zea mays）肌球蛋白XI蛋白，OPAQUE 1（O 1），是必需的不对称分裂在玉米气孔发育。我们分析了气孔前体细胞不对称分裂之前和期间，以确定为什么o 1突变体有异常的分裂面。细胞极化和核定位在o 1突变体中正常发生，并且正确指定了未来的分裂位点。在胞质分裂后期，当成膜体形成新生细胞板时，o 1的缺陷变得明显。在o 1中最初的成膜体引导是正常的;然而，随着成膜体继续扩张，o 1成膜体被误导。为了了解O 1如何有助于成膜体的指导，我们确定了O 1相互作用蛋白。玉米驱动蛋白与拟南芥分裂位点标记物成膜体定向驱动蛋白（POKs）相关，也是正确的成膜体指导所必需的，与O 1物理相互作用。我们建议，不同的肌球蛋白是重要的成膜体扩张的多个步骤，和O 1肌动蛋白电机和POK样微管电机一起工作，以确保正确的后期成膜体的指导。
Formative asymmetric divisions produce cells with different fates and are critical for development. We show the maize (Zea mays) myosin XI protein, OPAQUE1 (O1), is necessary for asymmetric divisions during maize stomatal development. We analyzed stomatal precursor cells before and during asymmetric division to determine why o1 mutants have abnormal division planes. Cell polarization and nuclear positioning occur normally in the o1 mutant, and the future site of division is correctly specified. The defect in o1 becomes apparent during late cytokinesis, when the phragmoplast forms the nascent cell plate. Initial phragmoplast guidance in o1 is normal; however, as phragmoplast expansion continues o1 phragmoplasts become misguided. To understand how O1 contributes to phragmoplast guidance, we identified O1-interacting proteins. Maize kinesins related to the Arabidopsis thaliana division site markers PHRAGMOPLAST ORIENTING KINESINs (POKs), which are also required for correct phragmoplast guidance, physically interact with O1. We propose that different myosins are important at multiple steps of phragmoplast expansion, and the O1 actin motor and POK-like microtubule motors work together to ensure correct late-stage phragmoplast guidance.