Early postnatal ethanol exposure has long-term effects on the performance of male rats in a delayed matching-to-place task in the Morris water maze
Early postnatal ethanol exposure has long-term effects on the performance of male rats in a delayed matching-to-place task in the Morris water maze
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DOI：
10.1097/00000374-200003000-00007
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发表时间：
2000-03-01
影响因子：
3.2
通讯作者：
Wainwright, PE
中科院分区：
文献类型：
--
作者：
Girard, TA;Xing, HC;Wainwright, PE
背景：有人担心，在没有全面发展的胎儿酒精综合征的情况下，怀孕期间酗酒可能会在后代中产生长期的认知缺陷。在这方面，空间工作记忆可能特别脆弱。这是首次在Morris水迷宫中使用延迟匹配位置(DMTP)任务来解决这一问题的动物模型。方法：新生雄性大鼠从出生后5天到18天进行胃造口术和人工饲养。从出生后6天到9天，它们在暴饮式暴露模型(BAC 302 mg/dl)中喂养6.5g·kg(-1).d(-1)乙醇(Etoh)或等热量麦芽糖-糊精溶液(MD)。这项研究包括第三个哺乳对照组(SC)，正常饲养。这些大鼠在DMTP任务中进行了一系列问题的测试，第一次是作为青少年(PD 35)，然后是两次作为成年。每个问题都包括最初的搜索试验和随后的试验试验。前两个测试阶段在这两个试验之间使用0秒或60秒的延迟。第三阶段将此延迟增加到60秒2小时。结果：在搜索实验后的第二次实验中，Etoh大鼠在重新定位隐藏平台的能力上比对照组受到了损害。在第一阶段和第二阶段，乙醇对0秒和60秒延迟条件下的性能没有不同的影响。然而，Etoh大鼠更容易受到阶段3中较长的2小时延迟的影响。在搜索实验、游泳速度和线索任务绩效上，两组之间没有差异。结论：这些发现表明，在大脑生长突发期过度暴露于乙醇会导致大鼠在水迷宫中的DMTP表现受到长期的损害。
Background: There is concern that, in the absence of full-blown fetal alcohol syndrome, binge drinking during pregnancy might produce long-term cognitive deficits in offspring. Spatial working memory might be particularly vulnerable in this regard. This is the first study to address this issue in an animal model of binge exposure during the brain growth spurt using a delayed matching-to-place (DMTP) task in the Morris water maze.Methods: Infant male rats were gastrostomized and reared artificially from postnatal days (PD) 5 to 18. From PD 6 to 9 they were fed either 6.5 g.kg(-1).d(-1) ethanol (EtOH) in a binge exposure model (BAC 302 mg/dl) or an isocaloric maltose-dextrin solution (MD). The study included a third suckled control group (SC) that was reared normally. The rats were tested on a series of problems in the DMTP task, first as juveniles (PD 35) and then twice again as adults. Each problem included an initial search trial and a subsequent test trial. The first two phases of testing used delays of either 0 sec or 60 sec between these two trials. The third phase increased this delay to 60 sec and 2 hr. In addition, the rats were tested on a cued task in the water maze.Results: EtOH rats were impaired relative to controls in their ability to relocate the hidden platform on the second trial, which followed the search trial. In Phases 1 and 2, there was no differential effect of ethanol on performance across the 0-sec and 60-sec delay conditions. However, EtOH rats were more affected by the longer 2-hr delay in Phase 3. There were no group differences on the search trial, in swimming speed, or cued-task performance.Conclusions: These findings establish that binge exposure to ethanol during the brain growth spurt results in a long-lasting impairment on the DMTP performance of rats in the water maze.