Thioalkalivibrio thiocyanoxidans sp nov and Thioalkalivibrio paradoxus sp nov., novel alkaliphilic, obligately autotrophic, sulfur-oxidizing bacteria capable of growth on thiocyanate, from soda lakes
Thioalkalivibrio thiocyanoxidans sp nov and Thioalkalivibrio paradoxus sp nov., novel alkaliphilic, obligately autotrophic, sulfur-oxidizing bacteria capable of growth on thiocyanate, from soda lakes
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DOI：
10.1099/00207713-52-2-657
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发表时间：
2002-03-01
影响因子：
2.8
通讯作者：
Kuenen, JG
中科院分区：
文献类型：
--
作者：
Sorokin, DY;Tourova, TP;Kuenen, JG
从西伯利亚东南部、肯尼亚和埃及的苏打湖中分离出9株嗜盐碱、专性自养、硫氧化细菌，它们能以硫氰酸盐（SCN-）为唯一能量和氮源生长，并以硫氰酸盐为底物，在pH 10的碳酸钠矿物缓冲液中富集。分离物分为两组，这两组在细胞形态、生长参数和氧化二硫化碳的能力方面有很大差异。细菌能够氧化硫化物，多硫化物，硫和连四硫酸盐，以及硫氰酸盐。两个菌株属于一个极端的halotolerant型生长在4 M Na+的存在下。氰尿酸（CNO-）是两组中硫氰酸盐降解过程中的主要含氮中间体。根据DNA-DNA杂交数据和系统发育分析，这两组菌株属于最近描述的一个属的haloalkaliphilic硫氧化细菌，即硫碱弧菌，属于γ-变形菌，其中他们代表两个新种。建议将具有桶形细胞的组命名为奇异硫碱弧菌（模式菌株ARh 1（T）= DSM 13531（T）= JCM 11367（T）），将具有弧菌形细胞的组命名为氧化硫氰硫碱弧菌（模式菌株ARh 2（T），DSM 13532（T）= JCM 11368（T））。根据新资料对硫碱弧菌属的诊断进行了修正。
Nine strains of haloalkaliphilic, obligately autotrophic, sulfur-oxidizing bacteria able to grow with thiocyanate (SCN-) as the sole energy and nitrogen source were isolated from soda lakes in South-East Siberia, Kenya and Egypt after enrichment on sodium carbonate minerals buffered at pH 10 with thiocyanate as the substrate. The isolates fell into two groups that were substantially different in terms of cell morphology, growth parameters and the ability to oxidize carbon disulfide. The bacteria were able to oxidize sulfide, polysulfide, sulfur and tetrathionate, as well as thiocyanate. Two isolates belonged to an extremely halotolerant type growing in the presence of up to 4 M Na+. Cyanate (CNO-) was the main nitrogen-containing intermediate during thiocyanate degradation in both groups. According to DNA-DNA hybridization data and phylogenetic analysis, both groups of isolates belong to a recently described genus of haloalkaliphillic sulfur-oxidizing bacteria, i.e. Thioalkalivibrio, belonging to the gamma-Proteobacteria, in which where they represent two new species. The species name Thioalkalivibrio paradoxus (type strain ARh 1(T) = DSM 13531(T) = JCM 11367(T)) is proposed for the group with barrel-shaped cells, and the species name Thioalkalivibrio thiocyanoxidans (type strain ARh 2(T), DSM 13532(T) = JCM 11368(T)) is proposed for the group with vibrio-shaped cells. The diagnosis of the genus Thioalkalivibrio is amended according to the new data.