Relative transcript abundance in porcine cumulus cells collected from different sized follicles
Relative transcript abundance in porcine cumulus cells collected from different sized follicles
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从不同大小的卵泡收集的猪卵丘细胞的相对转录本丰度
DOI：
10.1111/rda.13881
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发表时间：
2020
影响因子：
1.7
通讯作者：
Funahashi Hiroaki
中科院分区：
文献类型：
--
作者：
Moros‐Nicolas Carla;Izquierdo‐Rico Ma Jose;Li Yang;Gonzalez‐Brusi Leopoldo;Romar Raquel;Funahashi Hiroaki
卵母细胞与周围卵丘细胞之间的串扰是产生能态卵母细胞的必要条件。先前的研究分析了来自小（SF: <3毫米直径）和中等（MF: 3 - 6毫米直径）卵泡的卵母细胞的相对转录物丰度，以确定SF来源的卵母细胞在辅助生殖技术（ART）中的潜在用途。本研究的目的是检测从SF和MF衍生的卵母细胞复合物（COCs）中获得的CCs的相对转录物丰度。根据对发育能力的重要性选择了9个基因：富含AT的相互作用结构域1B （ARID1B）、骨形态发生蛋白受体2 （BMPR2）、CD44、促卵泡激素受体（FSHR）、卵泡抑制素（FST）、抑制素β - A （INHBA）、促黄体生成素受体（LHR）、核受体亚家族2组F成员6 （NR2F6）和血管内皮生长因子A （VEGFA）。RT - qPCR分析这些基因的表达。结果表明，在5个基因中存在显著差异，与MF来源的cc相比，SF来源的cc在INHBA中相对转录丰度较低，但在FSHR、FST、LHR和NR2F6中转录丰度较高。我们提供了来自不同大小卵泡的猪cc的基因活性信息，从而提高了我们对卵母细胞生物学的理解，并提供了识别活卵母细胞和能力卵母细胞的新标记。
Crosstalk between the oocyte and surrounding cumulus cells (CCs) is essential for the production of competent oocytes. Previous studies have analysed the relative transcript abundance in oocytes derived from small (SF: <3 mm diameter)‐ and medium‐sized (MF: 3–6 mm diameter) follicles to determine the potential use of SF‐derived oocytes in assisted reproductive technologies (ART). The aim of this study was to examine the relative transcript abundance of CCs obtained from cumulus–oocyte complexes (COCs) derived from SF and MF. Nine genes were selected according to their importance for developmental competence: AT‐rich interaction domain 1B (ARID1B), bone morphogenic protein receptor 2 (BMPR2), CD44, follicle‐stimulating hormone receptor (FSHR), follistatin (FST), inhibin beta‐A (INHBA), luteinizing hormone receptor (LHR), nuclear receptor subfamily 2 group F member 6 (NR2F6) and vascular endothelial growth factor A (VEGFA). The expression of these genes was analysed by RT‐qPCR. The results pointed to significant differences in five genes, and the relative transcript abundance of SF‐derived CCs was lower in the case of INHBA, but higher in FSHR, FST, LHR and NR2F6 compared with MF‐derived CCs. We provide information of gene activity in the porcine CCs from different sized follicles, thus improving our understanding of oocyte biology and providing new markers that identify viable and competent oocytes.