[Effect of acupuncture technique of Tiaoxin Tongdu on learning-memory ability and expressions of hippocampal VEGF and Ang-1 in rats with vascular dementia].
[Effect of acupuncture technique of Tiaoxin Tongdu on learning-memory ability and expressions of hippocampal VEGF and Ang-1 in rats with vascular dementia].
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DOI：
10.13703/j.0255-2930.20190821-0008
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发表时间：
2020-10-12
期刊：
影响因子：
--
通讯作者：
Tan, Tao
中科院分区：
文献类型：
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作者：
Gao, Yin-Lai;Tian, Hao-Mei;Tan, Tao
目的：观察调心通毒针法对血管性痴呆大鼠学习记忆能力及海马区血管内皮生长因子和血管生成素-1表达的影响，探讨调心通毒针法治疗血管性痴呆的作用机制。方法：24只雄性SD大鼠随机分为假手术组、模型组、药物组和Morris水迷宫实验后针刺组，每组6只。模型组、药物组、针刺组采用永久性结扎双侧颈总动脉的方法建立血管性痴呆模型。造模成功后次日给予治疗。针刺组大鼠针刺“百会”、“神庭”、“水沟”、“大椎”、“风府”、“名门”、“内关”、“大陵”、“老宫”穴30min，药物组给予尼莫地平溶液(0.0625 g/kg)灌胃，1次/d，共2周。在造模前、造模后2周、干预后用Morris水迷宫实验检测大鼠的行为，干预后用Western blot法检测大鼠海马区血管内皮生长因子和血管紧张素转换酶1的表达。结果：与假手术组相比，模型组大鼠平均逃避潜伏期延长(P
OBJECTIVE: To observe the effect of acupuncture technique of Tiaoxin Tongdu on learning-memory ability and expressions of hippocampal vascular endothelial growth factor (VEGF) and angiogenin-1 (Ang-1) in rats with vascular dementia (VD), and to explore the mechanism of acupuncture technique of Tiaoxin Tongdu for VD.METHODS: A total of 24 male SD rats were randomly divided into a sham operation group, a model group, a medication group and an acupuncture group after Morris water maze test, 6 rats in each group. VD model was established by permanent ligation of bilateral common carotid arteries in the model group, the medication group and the acupuncture group. Treatment was given on the next day after successful modeling. The rats in the acupuncture group were treated with acupuncture at "Baihui" (GV 20), "Shenting" (GV 24), "Shuigou" (GV 26), "Dazhui" (GV 14), "Fengfu" (GV 16), "Mingmen" (GV 4), "Neiguan" (PC 6), "Daling" (PC 7) and "Laogong" (PC 8) for 30 min; the rats in the medication group were treated with nimodipine solution (0.0625 g/kg) by gavage, once a day, for 2 weeks. Morris water maze test was used to detect the behavior of rats before modeling, 2 weeks after modeling and after intervention; after intervention, the expressions of VEGF and Ang-1 protein in hippocampus were detected by Western blot.RESULTS: Compared with the sham operation group, the average escape latency of rats in the model group was prolonged (P