Effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin suggests abnormal palate development after palatal fusion
Effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin suggests abnormal palate development after palatal fusion
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DOI：
10.1111/j.1741-4520.2010.00271.x
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发表时间：
2010-06-01
影响因子：
1.3
通讯作者：
Sugahara, Toshio
中科院分区：
文献类型：
--
作者：
Imura, Hideto;Yamada, Tomohiro;Sugahara, Toshio
暴露于2，3，7，8-四氯二苯并-对二恶英(TCDD)的小鼠胚胎会出现腭裂和肾积水。由于接触和/或融合失败，TCDD暴露后会发生腭裂。我们研究了融合后的腭突分离是否会导致腭裂的发生。将妊娠12天的小鼠随机分为两组，一组灌胃一剂橄榄油(对照组)，另一组灌胃一剂加橄榄油稀释的TCDD，剂量均为40mgg/kg体重。在妊娠小鼠的腭部形成期(GD13-18)，剖宫产取出胚胎，在立体显微镜下观察其腭部形态。在TCDD暴露的胚胎中，GD14、15和16出现了腭裂融合，GD18的腭裂发生率为100%，GD15和16的融合率分别为17.5+/-15.2%和12.4+/-11.8%。暴露于TCDD的小鼠胚胎的一些腭部在腭部形成过程中外侧腭突融合后显示出明显的腭裂。在TCDD暴露的小鼠胚胎中观察到大量的细胞，这些细胞主要是融合腭部的上皮细胞。观察到E-钙粘附素在这块细胞中的表达减少，表明它参与了腭裂的发展。
Mouse embryos exposed to 2,3,7,8-tetrachloridedibenzo-p-dioxin (TCDD) develop cleft palates and hydronephrosis. Cleft palates occur after TCDD exposure due to contact and/or fusion failure. We investigated whether cleft palate can be induced by dissociation of the palatine process after fusion. Pregnant mice on gestational day (GD) 12 were randomly divided into two groups: one group was administered through gastric tubes one dose of olive oil (control group) and the other group was administered one dose of TCDD diluted with olive oil, both at a dose of 40 mu g/kg body weight. Embryos were removed by cesarean section from pregnant mice during the palatal formation stage (GD 13-18) and the palatal form was observed using a stereoscopic microscope. In TCDD-exposed embryos, palatal fusion was observed on GD 14, 15 and 16 and the incidence of cleft palate was 100% on GD 18. Fusion rates were 17.5 +/- 15.2% and 12.4 +/- 11.8% on GD 15 and 16, respectively. Some palates from the TCDD-exposed mouse embryos showed clearly developed cleft palate after fusion of the lateral palatine processes during palatal formation. A mass of cells, which were chiefly epithelial in the fused palates was observed in the TCDD-exposed mouse embryos. A decrease in E-cadherin expression was observed in this mass of cells, indicating its involvement in the development of cleft palate.