A dicistronic gene pair within a cluster of "EF-hand" protein genes in the genomes of Drosophila species.
A dicistronic gene pair within a cluster of "EF-hand" protein genes in the genomes of Drosophila species.
复制标题
果蝇基因组中“EF-hand”蛋白基因簇内的双顺反子基因对。
DOI：
10.1016/j.ygeno.2006.04.009
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发表时间：
2006
期刊：
影响因子：
4.4
通讯作者：
Beckingham,KathleenM
中科院分区：
文献类型：
--
作者：
Pavlik,Paige;Konduri,Vanaja;Massa,Enrique;Simonette,Rebecca;Beckingham,KathleenM
Androcam 是一种果蝇钙调蛋白相关蛋白，专门在睾丸中发挥作用。我们证明 Acam 基因是以头尾相连的方式排列的三个无内含子基因簇的一部分。其他基因还编码具有睾丸特异性转录的钙调蛋白相关蛋白。 Acam 和最 5'端的基因 (gene1) 生成单顺反子转录本。令人惊讶的是，中心基因（gene2）仅被转录为与 Acam 的双顺反子转录物。在 D. yakuba 中也发现了类似的簇。在 D.pseudoobscura 中，该簇包含四个基因：两个 Acam 型基因，位于与基因 1 和基因 2 均相关的单个基因的下游，以及第四个弱相关基因。尽管如此，D.pseudoobscura簇也从类似于gene2-Acam对的基因对生成双顺反子转录本。共转录的gene1/2-Acam基因对可能是该基因座的基本特征。尽管 Acam 蛋白存在于黑腹果蝇和拟暗果蝇睾丸中，但免疫印迹和质谱技术无法检测到基因 1/2 型 ORF 的同源蛋白。
Androcam is a Drosophila melanogaster calmodulin-related protein that functions specifically in the testis. We show that the Acam gene is part of a cluster of three intronless genes arranged in a head-to-tail manner. The additional genes also encode calmodulin-related proteins with testis-specific transcription. Acam and the 5′-most gene (gene1) generate monocistronic transcripts. Surprisingly, the central gene (gene2) is transcribed only as a dicistronic transcript with Acam. A similar cluster is found in D. yakuba. In D. pseudoobscura, the cluster contains four genes: two Acam-type genes downstream of a single gene related to both gene1 and gene2 and a fourth weakly related gene. Nevertheless, the D. pseudoobscura cluster also generates a dicistronic transcript from a gene pair analogous to the gene2–Acam pair. A cotranscribed gene1/2–Acam gene pair may be the founding feature of this locus. Although Acam protein is present in D. melanogaster and D. pseudoobscura testes, cognate proteins for the gene1/2-type ORFs are not detectable by immunoblotting and mass spectrometry techniques.