A Self-Assembly Method for Creating Vascularized Tumor Explants Using Biomaterials for 3D Culture.
A Self-Assembly Method for Creating Vascularized Tumor Explants Using Biomaterials for 3D Culture.
复制标题
使用 3D 培养生物材料创建血管化肿瘤外植体的自组装方法。
DOI：
10.1007/978-1-0716-3056-3_12
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Bazou,Despina
中科院分区：
文献类型：
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作者：
Munn,LanceL;Bazou,Despina
Validation of potential therapeutic targets in cancer requires functional live assays that recapitulate the biology, anatomy, and physiology of human tumors. We present a methodology for maintaining mouse and patient tumor samples ex vivo for in vitro drug-screening as well as for the guidance of patient-specific chemotherapies. The harvested tumor biopsy, excised from mice or patients, is integrated into a support tissue that includes extended stroma and vasculature. The methodology is more representative than tissue culture assays, faster than patient-derived xenograft models, easy to implement, amenable to high-throughput assays and does not carry the ethical issues or expense associated with animal studies. Our physiologically relevant model can be successfully used for high-throughput drug screening.
