Impaired surfactant phospholipid metabolism in hyperoxic mouse lungs.
Impaired surfactant phospholipid metabolism in hyperoxic mouse lungs.
复制标题
高氧小鼠肺中表面活性剂磷脂代谢受损。
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
D. Smith
中科院分区：
文献类型：
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作者：
N. Gross;D. Smith
本文研究了98%O_2对小鼠肺磷脂含量、体内外合成速率的影响。通过多次支气管内灌洗获得的肺泡液显示，在48和72小时之间，所有磷脂的量均下降。二饱和磷脂酰胆碱的比例在96 h内没有变化。在同一时间发现肺组织的磷脂酰胆碱减少较小。在48和72小时之间的总磷脂酰胆碱和二饱和磷脂酰胆碱的营业额大大减少，从肺到肺泡液的渡越时间延长。在60小时，在体外掺入肺切片的甘油和棕榈酸酯到disaturated磷脂减少了约一半，标签到各种磷脂类的分布不变。我们解释这些数据支持的假设，即高氧损害肺表面活性剂磷脂的合成和运输到肺泡表面，这导致肺泡衬里层的磷脂含量减少。这些影响可以解释肺机械特性的改变，并导致O2中毒的发病率和死亡率。
We studied the effect of 98% O2 on the amounts of and in vivo and in vitro synthesis rates of lung phospholipids in the mouse. The alveolar fluid obtained by multiple endobronchial lavages showed a decline in the amounts of all phospholipids between 48 and 72 h. The proportion of phosphatidylcholine that was disaturated did not change up to 96 h. Smaller decreases in phosphatidylcholine of the lung tissue were found at the same times. Between 48 and 72 h the turnover of total and disaturated phosphatidylcholine was greatly decreased, and transit time for both from lung to alveolar fluid was prolonged. At 60 h in vitro incorporation by lung slices of both glycerol and palmitate into disaturated phospholipids was reduced by approximately half, the distribution of label into various phospholipid classes being unchanged. We interpret these data as supporting the hypothesis that hyperoxia impairs the synthesis of surfactant phospholipids in the lung and their transport to the alveolar surface, and that this results in a decrease in the phospholipid content of the alveolar lining layer. These effects could account for altered pulmonary mechanical properties and contribute to the morbidity and mortality of O2 toxicity.