Effect of Solvent-Co-solvent Mixtures on Lipase-Catalyzed Transesterification of Canola Oil
Effect of Solvent-Co-solvent Mixtures on Lipase-Catalyzed Transesterification of Canola Oil
复制标题
DOI：
10.1021/ef901176h
复制
发表时间：
2010-09-01
期刊：
影响因子：
5.3
通讯作者：
Vasudevan, Palligarnai T.
中科院分区：
文献类型：
--
作者：
Fu, Boyi;Vasudevan, Palligarnai T.
在这项工作中，建立了不同的溶剂-共溶剂混合物，以提高酶的活性和底物的传质在菜籽油的酶促酯交换反应中的假丝酵母脂肪酶催化。溶剂-共溶剂混合物包含疏水性溶剂和亲水性共溶剂。取决于共溶剂，在低共溶剂浓度下，由于甲醇在共溶剂介质中的分散的改善，生物柴油的产率提高。然而，在较高的共溶剂浓度下，由于脂肪酶的失活，生物柴油产率降低。将溶剂-助溶剂混合物的极性与生物柴油的产率相关联，以评估反应介质的极性与脂肪酶活性之间的潜在关系。研究了水含量对酯交换反应中脂肪酶活性的影响。
In this work, various solvent-co-solvent mixtures were established to enhance both enzyme activity and mass transfer of substrates in the enzymatic transesterification of canola oil catalyzed by Candida antarctic lipase. The solvent-co-solvent mixture comprised a hydrophobic solvent and a hydrophilic co-solvent. Dependent upon the co-solvent, at low co-solvent concentrations, the yield of biodiesel was enhanced because of the improvement in the dispersion of methanol in the co-solvent media. However, at higher co-solvent concentrations, the biodiesel yield was reduced because of the inactivation of lipase. The polarity of solvent-co-solvent mixtures was correlated with the yield of biodiesel to assess the potential relationship between the polarity of reaction media and lipase activity. The effect of the water content on lipase activity in the transesterification reaction was also ascertained.