A disposable chemical heater and dry enzyme preparation for lysis and extraction of DNA and RNA from microorganisms
A disposable chemical heater and dry enzyme preparation for lysis and extraction of DNA and RNA from microorganisms
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DOI：
10.1039/c6ay00107f
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发表时间：
2016-01-01
影响因子：
3.1
通讯作者：
Yager, P.
中科院分区：
文献类型：
--
作者：
Buser, J. R.;Zhang, X.;Yager, P.
样品制备，包括细菌裂解，仍然是实现对许多病原体进行完整的即时检测的障碍。在这里，我们开发了一种样品制备方法，用于酶解和样品加热，用于低资源，护理点应用。我们展示了一种无仪器的化学加热系统，用于快速裂解革兰氏阳性细菌（金黄色葡萄球菌）和RNA病毒（人类呼吸道合胞病毒），使用干燥的金黄色葡萄球菌裂解酶混合物（变色肽酶）。裂解步骤后(
Sample preparation, including bacterial lysis, remains a hurdle in the realization of complete point-of-care tests for many pathogens. Here, we developed a sample preparation methodology for enzymatic lysis and sample heating for low-resource, point-of-care applications. We show an instrument-free chemical heater system for rapid lysis of a Gram-positive bacterium (Staphylococcus aureus) and an RNA virus (human respiratory syncytial virus) using a dried lysis enzyme mixture (achromopeptidase) for S. aureus. After a lysis step (