Definition and interactions of a positive regulatory element of the Arabidopsis INNER NOOUTER promoter
Definition and interactions of a positive regulatory element of the Arabidopsis INNER NOOUTER promoter
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DOI：
10.1046/j.1365-313x.2003.01971.x
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发表时间：
2004-02-01
期刊：
影响因子：
7.2
通讯作者：
Gasser, CS
中科院分区：
文献类型：
--
作者：
Meister, RJ;Williams, LA;Gasser, CS
INNER NO OUTER（INO）基因的表达仅限于拟南芥胚珠外珠被起始和发育过程中的远轴细胞层。使用先前定义的INO启动子（P-INO）的缺失分析，至少有三个不同的区域，有助于内源性INO表达模式进行了鉴定。一个这样的阳性元件，命名为POS 9，它包含至少三个不同的子元件，被发现包括足够的信息，以复制INO表达模式时，四个或更多个拷贝与异源最小启动子结合使用。虽然已知的INO调节剂，包括INO，SUPERMAN，BELL 1和AINTEGUMENTA，在酵母单杂交测定中没有可检测到的与POS 9相互作用，但在单杂交文库筛选中鉴定了两组与POS 9特异性相互作用的蛋白质。一组成员包括C2 H2锌指基序。第二组的成员含有一个新的、保守的DNA结合区，并根据该区域的保守特征被命名为碱性五半胱氨酸（BPC）蛋白。BPC蛋白是核定位的，并且在体外特异性结合位于P0 S9内的GA二核苷酸重复。BPC的广泛表达模式和拟南芥基因组中大量的GA重复潜在靶序列表明BPC蛋白可能影响参与多种植物过程的基因的表达。
INNER NO OUTER (INO) expression is limited to the abaxial cell layer of the incipient and developing outer integument in Arabidopsis ovules. Using deletion analysis of the previously defined INO promoter (P-INO), at least three distinct regions that contribute to the endogenous INO expression pattern were identified. One such positive element, designated POS9, which comprises at least three distinct subelements, was found to include sufficient information to duplicate the INO expression pattern when four or more copies were used in conjunction with a heterologous minimal promoter. While known regulators of INO, including INO, SUPERMAN, BELL1, and AINTEGUMENTA, did not detectably interact with POS9 in yeast one-hybrid assays, two groups of proteins that interact specifically with POS9 were identified in one-hybrid library screens. Members of one group include C2H2 zinc finger motifs. Members of the second group contain a novel, conserved DNA-binding region and were designated the BASIC PENTACYSTEINE (BPC) proteins on the basis of conserved features of this region. The BPC proteins are nuclear localized and specifically bind in vitro to GA dinucleotide repeats located within POS9. The widespread expression patterns of the BPCs and the large number of GA repeat potential target sequences in the Arabidopsis genome indicate that BPC proteins may affect expression of genes involved in a variety of plant processes.