Selective immobilization of DNA and antibody probes on electrode arrays: Simultaneous electrochemical detection of DNA and protein on a single platform
Selective immobilization of DNA and antibody probes on electrode arrays: Simultaneous electrochemical detection of DNA and protein on a single platform
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DOI：
10.1021/la701775g
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发表时间：
2007-07-31
期刊：
影响因子：
3.9
通讯作者：
Brozik, Susan M.
中科院分区：
文献类型：
--
作者：
Harper, Jason C.;Polsky, Ronen;Brozik, Susan M.
描述了一种用于DNA和蛋白质在阵列电极上的电寻址共价固定以及同时检测同一样品溶液中的多个生物制剂的概念证明程序。通过偏压辅助组装，将羧基苯基重氮离子选择性地沉积到阵列中的九个可单独寻址的电极中的五个上。通过碳化二亚胺化学将胺功能化的DNA探针共价偶联到羧基表面。随后通过循环伏安法将重氮-抗体结合物共价固定到剩余的四个电极中。报道了同时检测乳腺癌BRCA1基因和人类细胞因子白介素12的DNA序列，白介素12是免疫系统反应和肿瘤细胞攻击的重要组成部分。这些结果证明了在单个设备上选择不同生物分子图案化的可能性，并可能对未来微阵列和生物传感器的发展具有重要意义。
A proof of concept procedure for the electroaddressable covalent immobilization of DNA and protein on arrayed electrodes along with simultaneous detection of multiple bioagents in the same sample solution is described. Carboxyphenyldiazonium was selectively deposited onto five of nine individually addressable electrodes in an array via bias assisted assembly. Amine functionalized DNA probes were covalently coupled to the carboxyl surface via carbodiimide chemistry. This was followed by the covalent immobilization of diazonium-antibody conjugates into the remaining four electrodes via cyclic voltammetry. Simultaneous electrochemical detection of a DNA sequence related to the breast cancer BRCA1 gene and the human cytokine protein interleukin-12, which is a substantial component in the immune system response and attack of tumor cells, is reported. These results demonstrate the possibility of selective patterning of diverse biomolecules on a single device and may have significant implications for future development of microarrays and biosensors.