Thermoresponsive hydrophobic copolymer grafted on agar microspheres for all-aqueous bioseparations
Thermoresponsive hydrophobic copolymer grafted on agar microspheres for all-aqueous bioseparations
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用于全水生物分离的琼脂微球上接枝的热响应疏水共聚物
DOI：
10.1002/jssc.201800922
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发表时间：
2019
影响因子：
3.1
通讯作者：
Tianwei Tan
中科院分区：
文献类型：
--
作者：
Rui Zhang;Yongqin Lv;Tianwei Tan
采用水-油乳化法制备琼脂微球，并在碱性条件下进行交联。通过原子转移自由基聚合将温敏性疏水共聚物聚（N-异丙基丙烯酰胺-co-甲基丙烯酸十二酯-co-丙烯酰胺）接枝到琼脂微球上。通过光学显微镜、元素分析、傅里叶变换红外光谱、扫描电子显微镜和X射线光电子能谱对接枝共聚物的琼脂微球进行了表征。不同温度下，接枝共聚物的链长和疏水单体比例对琼脂微球的疏水性和吸附能力有显著影响。将该温敏微球用于蛋白质的分离，在不改变移动的相组成的情况下，通过改变温度，该微球表现出结合和释放行为。因此，我们建议将温敏琼脂微球作为全水生物分离的替代分离介质。
Agar microspheres were prepared by water–oil emulsification and cross‐linked under alkaline condition. The thermoresponsive hydrophobic copolymer, poly(N‐isopropylacrylamide‐co‐lauryl methacrylate‐co‐acrylamide), was grafted on the agar microspheres via atom transfer radical polymerization. The agar microspheres grafted with copolymers were characterized by light microphotography, elemental analysis, Fourier transform infrared spectroscopy, scanning electron microscopy, and X‐ray photoelectron spectroscopy. The chain lengths and hydrophobic monomer ratio of the grafting linear polymer had significant effects on the hydrophobicity and adsorption capacity of agar microspheres at different temperatures. The thermoresponsive microspheres were used for separation of proteins and showed binding and release behavior by change of temperatures without change in mobile phase composition. Thus, we suggest thermoresponsive agar microspheres as an alternative separation media for all‐aqueous bioseparations.