Analysis of partner of inscuteable, a novel player of Drosophila asymmetric divisions, reveals two distinct steps in inscuteable apical localization
Analysis of partner of inscuteable, a novel player of Drosophila asymmetric divisions, reveals two distinct steps in inscuteable apical localization
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DOI：
10.1016/s0092-8674(00)80676-5
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发表时间：
2000-02-18
期刊：
影响因子：
64.5
通讯作者：
Chia, W
中科院分区：
文献类型：
--
作者：
Yu, FW;Morin, X;Chia, W
不对称定位是inscuteable（insc）协调和介导果蝇细胞不对称分裂的先决条件。我们在这里显示，合作伙伴的不可见（引脚），一个新的组成部分，不对称的部门，需要不可见的不对称本地化。在钉的情况下，Inscuteable成为细胞质和不对称分裂的神经母细胞和有丝分裂域9细胞显示缺陷，让人想起insc突变体。针与Insc共定位，并与指导其不对称定位所必需和足够的区域相互作用。在神经母细胞引脚功能的分析揭示了两个不同的步骤为Insc顶端皮质定位：引脚独立，火箭筒依赖的启动步骤在分层（间期）和后期的维护步骤，在此期间，巴兹，引脚，和Insc本地化是相互依存的。
Asymmetric localization is a prerequisite for inscuteable (insc) to function in coordinating and mediating asymmetric cell divisions in Drosophila. We show here that Partner of Inscuteable (Pins), a new component of asymmetric divisions, is required for Inscuteable to asymmetrically localize. In the absence of pins, Inscuteable becomes cytoplasmic and asymmetric divisions of neuroblasts and mitotic domain 9 cells show defects reminiscent of insc mutants. Pins colocalizes with Insc and interacts with the region necessary and sufficient for directing its asymmetric localization. Analyses of pins function in neuroblasts reveal two distinct steps for Insc apical cortical localization: A pins-independent, bazooka-dependent initiation step during delamination (interphase) and a later maintenance step during which Baz, Pins, and Insc localization are interdependent.