Regulatory B cells are inversely associated with disease activity in rheumatoid arthritis.
Regulatory B cells are inversely associated with disease activity in rheumatoid arthritis.
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DOI：
10.3349/ymj.2014.55.5.1354
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发表时间：
2014-09
影响因子：
2.4
通讯作者：
Lee JH
中科院分区：
文献类型：
--
作者：
Kim J;Lee HJ;Yoo IS;Kang SW;Lee JH
已知调节性B淋巴细胞的功能在炎性疾病中是异常的。然而，最近的研究表明，IL-10+ B细胞似乎在类风湿性关节炎（RA）中扩增。因此，研究了RA患者和健康对照者外周血中IL-10+ B细胞的状态。从RA患者和年龄和性别匹配的健康对照者的血液样品中纯化外周血单个核细胞中的CD 19+细胞，并用CD 40配体和CpG刺激48小时。然后，使用流式细胞术分析CD 19+细胞中的细胞内IL-10。10例RA患者和10例健康对照组的IL-10+ B细胞比例无显著差异（RA，0.300±0.07 vs.健康对照组0.459±0.07，p=0.114）。RA患者诱导的IL-10+ B细胞占总B细胞的比例显著高于对照组（RA，4.44±3.44% vs.健康对照组2.44± 1.64%，p=0.033）。然而，IL-10+ B细胞占总B细胞的比例与RA患者的疾病活动性呈负相关（r=-0.398，p=0.040）。红细胞沉降率或C反应蛋白或药物与IL-10+ B细胞的比例无关。RA患者IL-10+ B细胞比例较健康对照组升高，但与疾病活动度呈负相关。
The function of regulatory B lymphocytes is known to be abnormal in inflammatory diseases. However, a recent study indicates that IL-10+ B cells seem to be expanded in rheumatoid arthritis (RA). Therefore, the state of IL-10+ B cells in the peripheral blood from RA patients and healthy controls were investigated. CD19+ cells in peripheral blood mononuclear cells were purified from blood samples of RA patients and age and gender-matched healthy controls, and stimulated with CD40 ligand and CpG for 48 hours. Then, intracellular IL-10 in CD19+ cells was analyzed using flow cytometry. There was no significant difference in the proportion of IL-10+ B cells between 10 RA patients and 10 healthy controls (RA, 0.300±0.07 vs. healthy control 0.459±0.07, p=0.114). The proportion of induced IL-10+ B cells to total B cells in RA patients was significantly higher than those in controls (RA, 4.44±3.44% vs. healthy control 2.44±1.64%, p=0.033). However, the proportion of IL-10+ B cells to total B cells correlated negatively with disease activity in RA patients (r=-0.398, p=0.040). Erythrocyte sedimentation rate or C-reactive protein or medication was not associated with the proportion of IL-10+ B cells. The proportion of induced IL-10+ B cell increased in RA patients compared to healthy control, however, negatively correlated with disease activity in RA.
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影响因子：
6
作者：
Yang, Min;Deng, Jun;Lu, Liwei
通讯作者：
Lu, Liwei
DOI：
10.1084/jem.184.6.2271
发表时间：
1996-12-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Wolf SD;Dittel BN;Hardardottir F;Janeway CA Jr
通讯作者：
Janeway CA Jr
影响因子：
32.4
作者：
Yanaba, Koichi;Bouaziz, Jean-David;Tedder, Thomas F.
通讯作者：
Tedder, Thomas F.
影响因子：
4.4
作者：
Evans, Jamie G.;Chavez-Rueda, Karina A.;Mauri, Claudia
通讯作者：
Mauri, Claudia
影响因子：
3.5
作者：
Liu, MF;Chao, SC;Lei, HY
通讯作者：
Lei, HY