Detection of drug resistance in human tumors by in vitro enzymatic amplification.
Detection of drug resistance in human tumors by in vitro enzymatic amplification.
复制标题
通过体外酶扩增检测人类肿瘤的耐药性。
作者：
M. Kashani;J. Rossi;Yani Lu;J. Ma;Jianjun Chen;H. Miyachi;K. Scanlon
已证明对化疗药物的获得性耐药性和天然耐药性在肿瘤的治疗中存在问题。胸苷酸合酶催化胸苷前体的合成，已被证明在多种化疗药物的作用下会被扩增。这种扩增的检测可能有益于替代临床方案的开发。在这项研究中，我们报告了使用现有的酶扩增方法来检测顺铂抗性时胸苷酸合酶基因的早期扩增。该测定利用聚合酶链式反应的改进，其中胸苷酸合酶基因的序列被扩增，包括两个侧翼寡核苷酸，充当DNA合成的引物。该方法比传统的核酸检测方法具有更高的灵敏度，并且需要少于 100 ng 的患者肿瘤总 RNA，并且无需体外培养患者细胞。
Both acquired and natural resistance to chemotherapy agents have proved problematic in the treatment of neoplasia. Thymidylate synthase, which catalyzes the synthesis of thymidine precursors, has been shown to be amplified in response to a variety of chemotherapeutic agents. The detection of such amplification could prove beneficial in the development of alternative clinical protocols. In this study we report the use of existing enzymatic amplification methods in order to detect incipient amplification of the thymidylate synthase gene upon resistance to cisplatin. The assay utilizes a modification of the polymerase chain reaction in which a sequence of the thymidylate synthase gene is amplified including two flanking oligonucleotides acting as primers for DNA synthesis. This method exhibits greater sensitivity than conventional nucleic acid detection methods and requires less than 100 ng of total RNA from patient tumors and no in vitro culturing of patient cells.
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DOI：
10.1073/pnas.83.23.8923
发表时间：
1986-12-01
影响因子：
11.1
作者：
SCANLON, KJ;NEWMAN, EM;PRIEST, DG
通讯作者：
PRIEST, DG
影响因子：
56.9
作者：
SLAMON, DJ;CLARK, GM;MCGUIRE, WL
通讯作者：
MCGUIRE, WL
影响因子：
3.2
作者：
Levine, Andrew J.;Reynolds, Sandra;Cox, Christopher;Miller, Eric N.;Sinsheimer, Janet S.;Becker, James T.;Martin, Eileen;Sacktor, Ned
通讯作者：
Sacktor, Ned
人卵巢癌细胞体内外顺铂-FUra 协同作用和顺铂耐药机制。
影响因子：
--
作者：
Scanlon,KJ;Lu,Y;Kashani-Sabet,M;Ma,J;Newman,E
通讯作者：
Newman,E