A novel bioactive membrane by cell electrospinning
A novel bioactive membrane by cell electrospinning
复制标题
通过细胞静电纺丝制备新型生物活性膜
DOI：
10.1016/j.yexcr.2015.08.007
复制
发表时间：
2015-11-01
影响因子：
3.7
通讯作者：
Hu, Qingxi
中科院分区：
文献类型：
--
作者：
Chen, Haiping;Liu, Yuanyuan;Hu, Qingxi
静电纺丝可以制造出与天然细胞外基质的规模和机械行为相似的生物可降解基质。然而，用传统技术实现高细胞密度和细胞在基质中的渗透仍然具有挑战性和耗时。本文提出的细胞静电纺丝技术可以缓解与这些限制相关的问题。在细胞电纺技术中，用该材料包裹的细胞存活良好，均匀分布在纳米纤维膜中，培养28d后细胞活力提高到133%。电纺纳米纤维膜具有一定的降解性和良好的细胞-膜相互作用，支持膜的活性生物相容性。它的特性有助于支持细胞的附着和生长，保持表型形状，并分泌大量的细胞外基质(ECM)。这种新型膜在组织工程领域具有潜在的应用前景。细胞电纺将细胞微集成为可生物降解的纤维基质的能力体现了一种新的组织工程方法，可以应用于构建高细胞密度的弹性组织模拟物。(C)2015 Elsevier Inc.保留所有权利。
Electrospinning permits fabrication of biodegradable matrices that can resemble the both scale and mechanical behavior of the native extracellular matrix. However, achieving high-cellular density and infiltration of cells within matrices with traditional technique remain challenging and time consuming. The cell electrospinning technique presented in this paper can mitigate the problems associated with these limitations. Cells encapsulated by the material in the cell electrospinning technique survived well and distributed homogenously within the nanofibrous membrane, and their vitality was improved to 133% after being cultured for 28 days. The electrospun nanofibrous membrane has a certain degradation property and favorable cell-membrane interaction that supports the active biocompatibility of the membrane. Its properties are helpful for supporting cell attachment and growth, maintaining phenotypic shape, and secreting an ample amount of extracellular matrix (ECM). This novel membrane may be a potential application within the field of tissue engineering. The ability of cell electrospinning to micro-integrate cells into a biodegradable fibrous matrix embodies a novel tissue engineering approach that could be applied to fabricate a high cell density elastic tissue mimetic. (C) 2015 Elsevier Inc. All rights reserved.