Comparison of the immune responses induced by local immunizations with recombinant Lactobacillus plantarum producing tetanus toxin fragment C in different cellular locations
Comparison of the immune responses induced by local immunizations with recombinant Lactobacillus plantarum producing tetanus toxin fragment C in different cellular locations
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DOI：
10.1016/s0264-410x(02)00027-0
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发表时间：
2002-03-15
期刊：
影响因子：
5.5
通讯作者：
Mercenier, A
中科院分区：
文献类型：
--
作者：
Reveneau, N;Geoffroy, MC;Mercenier, A
选择植物乳杆菌NCIMB 8826作为用于开发活粘膜疫苗的细菌载体。据报告，该菌株在饲喂人类志愿者时显示出有趣的药代动力学特性，并且还能够在小鼠肠道中持续存在。以无毒的破伤风毒素C片段（TTFC）为模型抗原，通过皮下、鼻内和灌胃途径免疫小鼠，比较了在细胞内、分泌和细胞表面暴露三种细胞位置产生TTFC的重组菌株。三种类型的构建体能够通过所有测试的途径诱导针对TTFC的强特异性免疫应答。虽然细胞表面呈递需要较低的抗原剂量才具有免疫原性，但在细胞质中产生大量TTFC的菌株获得了最高的IgG血清抗体滴度。(C)2002爱思唯尔科技有限公司。保留所有权利。
Lactobacillus plantarum NCIMB8826 was selected as a bacterial carrier for the development of live mucosal vaccines. This strain was reported to display interesting pharmaco-kinetic properties when fed to human volunteers and is also able to persist in the mouse intestine. The non-toxic C fragment of tetanus toxin (TTFC) was used as a model antigen, Recombinant strains producing TTFC in three cellular locations, intracellular, secreted or cell-surface exposed were compared to each other by immunizing mice through the subcutaneous, intranasal and intragastric routes. The three types of constructs were able to induce strong specific immune responses against TTFC by all routes tested. While cell-surface presentation required lower antigen doses to be immunogenic, the highest IgG serum antibody titers were obtained with the strain producing large amounts of TTFC in the cytoplasm. (C) 2002 Elsevier Science Ltd. All rights reserved.