Characterization of Listeria monocytogenes isolates in import food products of China from 8 provinces between 2005 and 2007.
Characterization of Listeria monocytogenes isolates in import food products of China from 8 provinces between 2005 and 2007.
复制标题
DOI：
10.1111/j.1750-3841.2011.02596.x
复制
发表时间：
2012-04
影响因子：
3.9
通讯作者：
Ping Wang;Hairong Yang;Yue Hu;Fei Yuan;Guiming Zhao;Yongsheng Zhao;Ying Chen
中科院分区：
文献类型：
--
作者：
Ping Wang;Hairong Yang;Yue Hu;Fei Yuan;Guiming Zhao;Yongsheng Zhao;Ying Chen
对2005年至2008年从我国8个省份进口的48株单核细胞增生李斯特菌进行了分离鉴定。确定各菌株的血清型和毒力，并采用多位点序列分型（MLST）进行分子分型。其中25株为1/2a型，11株为1/2b型，7株为4 b型，5株为1/2c、4a和4 e型。分子分型方法在这些分离株中发现了32种序列型（ST），大多数L。单核细胞增多症Ⅱ系占56%，其次为Ⅰ系占38%，Ⅲ系占6%。两个分子亚型簇，簇A包括谱系II的所有分离株，而簇B包含谱系I和谱系III的分离株。两个湖单核细胞增多性菌株没有被分组到两个簇中的任何一个中。53个菌株的毒力与L.单核细胞增多症参考株EGD对小鼠的毒力较强，而22213和22265对小鼠的毒力较弱。这些结果首次提供了对L.从我国8个省的进口食品中分离到的单核细胞增多症菌株表明，如果摄入受污染的食品，可能会引起人类疾病。MLST可作为L.单核细胞增生分离株。我国应加强对进口食品的检验检疫工作。实际应用在中国存在着潜在的猪链球菌病的危险，常规的L。单核细胞增多症分离株是重要的。加强对进口食品的监督管理是食品卫生管理的需要。
UNLABELLED A total of 48 Listeria monocytogenes isolates of different import food products from 8 provinces between 2005 and 2008 were characterized. The serotype and virulence were confirmed for each strain and molecular subtyping were analyzed by multilocus sequence typing (MLST). Twenty five strains were assigned to serotype 1/2a, and 11 isolates to serotype 1/2b, serotype 4b were found in 7 isolate, and the remaining 5 strains were grouped into serotypes 1/2c, 4a, and 4e. Molecular subtyping schemes found thirty two sequence types (STs) among these isolates and the majority of L. monocytogenes strains belonged to lineage II (56%), followed by lineage I (38%), lineage III (6%). Two molecular subtype clusters, cluster A included all isolates of lineage II, while cluster B contained the isolates of lineages I and lineages III. Two L. monocytogenes strains were not grouped in either of the two clusters. Fifty three isolates were as virulent as L. monocytogenes reference strain EGD in mouse virulence assay, while the isolates 22213 and 22265 had low pathogenicity. These results provide the first molecular insight into the L. monocytogenes strains isolated from import food products of 8 provinces in China and indicate the potential risk to cause human disease if intake by contaminated foods. MLST could be used as a routine subtyping method of L. monocytogenes isolates. In China, inspection and quarantine strategies of imported foods should be strengthened. PRACTICAL APPLICATION There is a potential risk of listeriosis in China and routine subtyping of L. monocytogenes isolates is important. It is necessary for food hygiene management to strengthen the supervision of imported foods.