Enhanced bactericidal toxicity of silver nanoparticles by the antibiotic gentamicin
Enhanced bactericidal toxicity of silver nanoparticles by the antibiotic gentamicin
复制标题
抗生素庆大霉素增强纳米银的杀菌毒性
DOI：
10.1039/c6en00031b
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Haifang Wang
中科院分区：
文献类型：
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作者：
Yan Wen Wang;Huan Tang;Di Wu;Dong Liu;Yuanfang Liu;Aoneng Cao;Haifang Wang
环境中纳米材料性质的改变可能会改变nano-.bio界面的相互作用和相应的生物反应。在这项研究中，我们发现抗生素庆大霉素显着增强聚（N-乙烯基-2-吡咯烷酮）包裹的银纳米颗粒的抗菌活性。通过改变培养基中银纳米颗粒的性质，银PVP纳米粒对三种细菌（S.金黄色葡萄球菌E.大肠杆菌和庆大霉素耐药大肠杆菌。根据抑菌分数指数、细菌存活率和生长曲线的数据，对大肠杆菌的抑菌效果进行了分析。彻底的研究表明，庆大霉素显著地促进Ag-PVP NPs的溶解，这不仅增加了系统中银离子的浓度，而且通过减轻NPs的负电荷而促进Ag-PVP NPs附着到细菌表面。相应的顺序是增强的细菌生长抑制和死亡。同时，令人惊讶地发现庆大霉素也与溶解的银离子结合，然后抑制银离子的抗菌活性。换句话说，庆大霉素在Ag-PVP NP的抗菌活性中起双重作用。该研究对于理解纳米粒子在复杂体系中的生物效应具有重要意义。
The alteration of properties of nanomaterials in the environment may change the interaction of the nano–.bio interface and the corresponding bio-responses. In this study, we find that the antibiotic gentamicin significantly.enhances the antibacterial activities of polyIJN-vinyl-2-pyrrolidone) coated silver nanoparticles.(Ag-PVP NPs) by altering the properties of silver nanoparticles in culture media. The enhanced antibacterial.activities of Ag-PVP NPs against three bacterial strains (S. aureus, E. coli and gentamicin-resistant E. coli).are concluded according to the data of the fractional inhibitory concentration indices, bacterial survival.rates and growth curves. A thorough investigation indicates that gentamicin promotes the dissolution of.Ag-PVP NPs dramatically, which not only increases the concentration of silver ions in the system but also.facilitates the attachment of Ag-PVP NPs onto the surface of bacteria by mitigating the negative charge of.the NPs. The corresponding sequence is the augmented bacterial growth inhibition and death. Meanwhile,.it is surprisingly found that gentamicin also binds with the dissolved silver ions and then inhibits the antibacterial.activity of silver ions. In other words, gentamicin plays a dual role in the antibacterial activity of.Ag-PVP NPs. This study is valuable for understanding the bioeffects of nanoparticles in a complex system.