Deregulated Expression of the Per1 and Per2 in Human Gliomas
Deregulated Expression of the Per1 and Per2 in Human Gliomas
复制标题
人类神经胶质瘤中 Per1 和 Per2 的表达失调
DOI：
10.1017/s031716710001026x
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发表时间：
2010-05-01
影响因子：
3
通讯作者：
Wang, Fan
中科院分区：
文献类型：
--
作者：
Xia, He-chun;Niu, Zhan-feng;Wang, Fan
背景资料：越来越多的证据表明，生物钟失调在恶性肿瘤（包括胶质瘤）的发生发展中起着重要作用。然而，控制神经胶质瘤细胞昼夜节律的基因的分子机制还没有被探索。研究方法：利用逆转录聚合酶链反应和免疫组织化学技术，我们研究了两个重要的时钟基因，Per 1和Per 2，在33个胶质瘤的表达。结果：在33例胶质瘤中，28例Per 1阳性，23例Per 2阳性。Pert和Per 2在胶质瘤细胞中的表达水平与周围非胶质瘤细胞相比差异有统计学意义（P0. 05）。Per 2在高级别胶质瘤和低级别胶质瘤中的表达强度无差异（r=-0.330，P =0.061），而Pert在高级别胶质瘤中的表达水平明显低于低级别胶质瘤（r=-0.433，P =0.012）。结论：Pert和Per 2在胶质瘤细胞中的表达明显低于其周围的非胶质瘤细胞。因此。我们认为Pert和Per 2表达的紊乱可能导致正常昼夜节律控制的中断，从而有利于胶质瘤细胞的存活。生物钟基因在胶质瘤和非胶质瘤细胞中的差异表达可能为胶质瘤的化疗提供分子基础。
Background: Growing evidence shows that the deregulation of the circadian clock plays an important role in the development of malignant tumors, including gliomas. However, the molecular mechanisms of genes controlling circadian rhythm in glioma cells have not been explored. Methods: Using reverse transcription polymerase chain reaction and immunohistochemistry techniques, we examined the expression of two important clock genes, Per1 and Per2, in 33 gliomas. Results: In this study, out of 33 gliomas, 28 were Per1-positive, and 23 were Per2-positive. The expression levels of Pert and Per2 in glioma cells were significantly different from the surrounding non-glioma cells (P0.05). While there was no difference in the intensity of immunoactivity for Per2 between high-grade gliomas and low-grade gliomas (r=-0.330, P=0.061), the expression level of Pert in high-grade gliomas was significantly lower than that in low-grade gliomas(r=-0.433, P=0.012). Conclusions: In this study, we found that the expression of Pert and Per2 in glioma cells was much lower than in the surrounding non-glioma cells. Therefore. we suggest that disturbances in Pert and Per2 expression may result in the disruption of the control of normal circadian rhythm, thus benefiting the survival of glioma cells. Differential expression of circadian clock genes in glioma and non-glioma cells may provide a molecular basis for the chemotherapy of gliomas.