INSTABILITY OF DICENTRIC PLASMIDS IN YEAST
INSTABILITY OF DICENTRIC PLASMIDS IN YEAST
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DOI：
10.1073/pnas.80.1.228
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
DAVIS, RW
中科院分区：
文献类型：
--
作者：
MANN, C;DAVIS, RW
Dicentric plasmids containing either 2 copies of centromere 4 or 1 copy of centromere 4 and 1 copy of centromere 3 in the yeast plasmid vector YRp17 were constructed in vitro and introduced into yeast cells by DNA transformation. The resulting colonies were heterogeneous for a mixed population of rearranged plasmids. The rearrangements always involved deletion of 1 or both centromere sequences originally present on the plasmid. Heterogeneity was due to the continued production of deleted plasmids from a pool of unrearranged dicentric plasmids maintained within some of the yeast cells in the colony. The RAD52 gene product is known to be required for the repair of DNA double-strand breaks in yeast. Transformation of rad52 mutant yeast cells with dicentric plasmids gave rearranged plasmids similar to those observed with RAD+ yeast cells, but the transformation frequency was only 5-10% compared to transformation with monocentric plasmids. The ratio of unrearranged dicentric plasmid to deleted plasmids was greatly reduced in the rad52-transformed cells. These observations are consistent with a model in which centromeric DNA sequences can interact independently with the yeast cell spindle apparatus. Occasional movement of centromeres to opposite poles may result in mechanical breakage of plasmid sequences. Plasmids deleted for 1 or both centromere sequences can be obtained from these broken molecules and are resistant to further rearrangement.
