High-performance liquid chromatographic determinations of disaccharides resulting from enzymatic degradation of isomeric chondroitin sulfates.
High-performance liquid chromatographic determinations of disaccharides resulting from enzymatic degradation of isomeric chondroitin sulfates.
复制标题
高效液相色谱法测定异构硫酸软骨素酶降解产生的二糖。
DOI：
10.1016/0003-2697(79)90814-5
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发表时间：
1979
影响因子：
2.9
通讯作者：
H. Tieckelmann
中科院分区：
文献类型：
--
作者：
G. Lee;H. Tieckelmann
本文研究了Whatman Partisil-10 PAC和Altex LiChrosorb NH_2键合相色谱柱对硫酸软骨素异构体用软骨素酶消化后得到的双糖的分离。酶降解混合物中的取代不饱和二糖在两种键合固定相上进行快速等度分离。一个完整的分离可以在10分钟内建立。这些程序的发展加快了异构硫酸软骨素的酶促研究。基于定量高效液相色谱分离和二糖产物检测，研究了硫酸软骨素通过软骨素酶作用的解聚速率。
The use of Whatman Partisil-10 PAC and Altex LiChrosorb NH2bonded phase columns for the high-performance liquid chromatographic separation of the disaccharides obtained from digestion of isomeric chondroitin sulfates with chondroitinases has been investigated. The substituted unsaturated disaccharides in the enzymatic degradation mixtures undergo rapid isocratic separations on both bonded stationary phases. A complete separation can be established within 10 min. The development of these procedures has expedited enzymatic studies of isomeric chondroitin sulfates. The rate of depolymerization of chondroitin sulfates by the action of chondroitinases based on quantitative high-performance liquid chromatographic separations and detection of the disaccharide products was studied.