The relationship between NADPH-dependent lipid peroxidation and degradation of cytochrome P-450 in adrenal cortex mitochondria.
The relationship between NADPH-dependent lipid peroxidation and degradation of cytochrome P-450 in adrenal cortex mitochondria.
复制标题
NADPH依赖性脂质过氧化与肾上腺皮质线粒体细胞色素P-450降解之间的关系。
DOI：
10.1016/0006-291x(83)91186-5
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发表时间：
1983
影响因子：
3.1
通讯作者：
Kimura,T
中科院分区：
文献类型：
--
作者：
Klimek,J;Schaap,AP;Kimura,T
已在牛肾上腺皮质线粒体中研究了 NADPH 依赖性脂质过氧化与细胞色素 P-450 降解之间的关系。丙二醛的形成伴随着细胞色素 P-450 总含量的相应减少。脂质过氧化抑制剂还可以防止细胞色素 P-450 的损失，进一步证明 NADPH 依赖性脂质过氧化与 P-450 降解之间的直接关系。为了区分细胞色素 P-45011β 和 P-450scc，使用类固醇诱导的差异光谱来评估 P-450 降解。这些测量结果提供了第一个证据，表明两种 P-450 在 NADP 依赖性脂质过氧化过程中都会被降解，而 P-45011β 更容易受到此过程的影响。
The relationship between NADPH-dependent lipid peroxidation and the degradation of cytochrome P-450 has been studied in bovine adrenal cortex mitochondria. Malondialdehyde formation is accompanied by a corresponding decrease in total cytochrome P-450 content. Inhibitors of lipid peroxidation also prevent the loss of cytochrome P-450, further demonstrating a direct relationship between NADPH-dependent lipid peroxidation and degradation of P-450. To differentiate between cytochrome P-45011βand P-450scc, steroidinduced difference spectra were used to evaluate P-450 degradation. These measurements provide the first evidence that both P-450's are degraded during NADP-dependent lipid peroxidation with P-45011βbeing much more susceptible to this process.