Antibody Repertoire Development in Fetal and Neonatal Piglets. XX. B Cell Lymphogenesis Is Absent in the Ileal Peyer's Patches, Their Repertoire Development Is Antigen Dependent, and They Are Not Required for B Cell Maintenance
Antibody Repertoire Development in Fetal and Neonatal Piglets. XX. B Cell Lymphogenesis Is Absent in the Ileal Peyer's Patches, Their Repertoire Development Is Antigen Dependent, and They Are Not Required for B Cell Maintenance
复制标题
DOI：
10.4049/jimmunol.1101871
复制
发表时间：
2011-11-15
影响因子：
4.4
通讯作者：
Francis, David H.
中科院分区：
文献类型：
--
作者：
Butler, John E.;Santiago-Mateo, Kristina;Francis, David H.
绵羊的连续回肠派伊尔集合淋巴结（IPP）被认为是一种哺乳动物的囊等价物，其中B细胞以不依赖Ag的方式多样化其库。在解剖学和发育学上类似的IPP发生在猪身上。切除仔猪出生时约90%的IPP不会改变血清和分泌物中的IG水平，也不会延迟隔离器中的动物和确定的肠道植物群定植时Ab库的多样化。切除术或假手术升高血清中的IgG和伊加，并在灌洗液从肠道，肺，和唾液中。未观察到脾脏和外周淋巴结中含IgG、伊加和IgM细胞的频率发生变化。使用量化VDJ库多样化的指数，在缺乏IPP的仔猪和定殖对照组之间的三种次级淋巴组织中没有观察到差异，而两组显示出比晚期胎仔猪或保持无菌的仔猪多10倍的多样化。体细胞超突变在胎儿IPP和无菌仔猪IPP中非常低，但在定植后增加3至5倍。IPP中D-J信号关节环未恢复，V-DJ信号关节环比骨髓中低5倍，与胸腺和脾脏中的信号关节环相似。我们的结论是，猪IPP不是B细胞淋巴发生的网站，不经历Ag-独立的剧目多样化，并不是主要的淋巴组织，因为它们不需要维持血清和分泌物中的IG水平。免疫学杂志，2011，187：5141-5149。
The continuous ileal Peyer's patches (IPP) of sheep are regarded as a type of mammalian bursal equivalent where B cells diversify their repertoire in an Ag-independent fashion. Anatomically and developmentally similar IPP occur in swine. Resection of similar to 90% of the IPP in piglets at birth did not alter Ig levels in serum and secretions or retard diversification of the Ab repertoire when animals were maintained in isolators and colonized with a defined gut flora. Resection or sham surgery elevated IgG and IgA in serum and in lavage fluid from the gut, lung, and in saliva. No changes in the frequency of IgG-, IgA-, and IgM-containing cells in the spleen and peripheral lymph node were observed. Using an index that quantifies diversification of the VDJ repertoire, no differences were seen in three secondary lymphoid tissues between piglets lacking IPP and colonized controls, whereas both groups displayed > 10-fold greater diversification than did late-term fetal piglets or piglets maintained germ-free. Somatic hypermutation was very low in fetal IPP and the IPP of germ-free piglets but increased 3- to 5-fold after colonization. D-J signal joint circles were not recovered in IPP, and V-DJ signal joint circles were 5-fold lower than in bone marrow and similar to those in thymus and spleen. We conclude that the porcine IPP are not a site of B cell lymphogenesis, do not undergo Ag-independent repertoire diversification, and are not primary lymphoid tissue since they are not required for maintenance of Ig levels in serum and secretions. The Journal of Immunology, 2011, 187: 5141-5149.