Construction and immunological evaluation of recombinant Lactobacillus plantarum expressing SO7 of Eimeria tenella fusion DC-targeting peptide
Construction and immunological evaluation of recombinant Lactobacillus plantarum expressing SO7 of Eimeria tenella fusion DC-targeting peptide
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表达柔嫩艾美球虫融合 DC 靶向肽 SO7 的重组植物乳杆菌的构建及免疫学评价
DOI：
10.1016/j.vetpar.2017.01.023
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发表时间：
2017
影响因子：
2.6
通讯作者：
Wang Chunfeng
中科院分区：
文献类型：
--
作者：
Yang Guilian;Yao Jiayun;Yang Wentao;Jiang Yanlong;Du Jinfen;Huang Haibin;Gu Wei;Hu Jingtao;Ye Liping;Shi Chunwei;Shan Baolong;Wang Chunfeng
由柔嫩艾美耳球虫（球虫）和其他物种引起的球虫病是影响全球家禽养殖业的严重寄生虫病。乳杆菌菌株表现出许多特性，使它们成为有吸引力的候选者，作为将具有药学意义的化合物（特别是疫苗）递送至粘膜的递送载体。本研究构建了重组植物乳杆菌（共表达SO7和DCpep基因），并对其抗大肠杆菌的功效进行了研究。本研究对 tenellachallenge 进行了评估。肉鸡用活的重组L进行口服免疫。 plantarumNC8-pSIP409-SO7-DCpep 两周，然后用每只鸡 5 × 104E.tenellasporulated 卵囊进行攻击。在实验过程中，评估了体重增加、盲肠病变评分、粪便卵囊脱落以及血清和肠洗液中的抗体反应，作为保护性免疫的衡量标准。结果表明，鸡用重组活L.与对照组相比，植物乳可以增加体重增加和血清抗体反应。同时，免疫组粪便卵囊脱落显着减少（p < 0.01）。此外，重组L.根据病变评分和组织病理学盲肠切片，plantarum 可以显着减轻盲肠的病理损伤（p < 0.01）。因此，这些结果表明重组L. plantarumNC8-pSIP409-SO7-DCpep 可能成为一种有前景的口服大肠杆菌疫苗候选者。柔嫩感染。
The coccidiosis caused by Eimeria tenella (coccidian) and other species is a serious parasitic disease that affects the global poultry breeding industry. Lactobacillus strains exhibit a number of properties that make them attractive candidates as delivery vehicles for presentation to the mucosa of compounds with pharmaceutical interest, particularly vaccines. Here, the recombinant Lactobacillus plantarum (co-expressing SO7 and DCpep gene) was constructed, and its efficacy againstE. tenellachallenge was evaluated in this study. Broiler chickens were orally immunized with live recombinantL. plantarumNC8-pSIP409-SO7-DCpep for two weeks and were then challenged with 5 × 104E.tenellasporulated oocysts per chicken. During the experiment, body weight gains, cecum lesion scores, fecal oocyst shedding and antibody responses in serum and intestinal washes were assessed as measures of protective immunity. The results indicated that chickens immunized with live recombinantL. plantarumcan increase body weight gains and serum antibody responses compared to the control groups. Meanwhile, fecal oocyst shedding in the immunized group was significantly reduced (p < 0.01). Moreover, recombinantL. plantarumcan significantly relieve pathological damage in cecum, according to lesion scores and histopathologic cecum sections (p < 0.01). Therefore, these results indicate that recombinantL. plantarumNC8-pSIP409-SO7-DCpep could become a promising oral vaccine candidate againstE. tenellainfection.