Whole genome characterization of new bovine rotavirus G21P[29] and G24P[33] strains provides evidence for interspecies transmission
Whole genome characterization of new bovine rotavirus G21P[29] and G24P[33] strains provides evidence for interspecies transmission
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DOI：
10.1099/vir.0.028175-0
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发表时间：
2011-04-01
影响因子：
3.8
通讯作者：
Sugiyama, Makoto
中科院分区：
文献类型：
--
作者：
Abe, Masako;Ito, Naoto;Sugiyama, Makoto
我们报告了一种新的牛轮状病毒AzuK-1（G21 P [29]）毒株，从无症状小牛中分离。2007年，我们从日本兵库县的一头无症状奶牛中分离出另一种牛轮状病毒Dai-10株，该病毒具有轮状病毒分类工作组（RCWG）指定的新G24 P [33]基因型。为了深入了解这些菌株的起源和进化，我们确定了这两种菌株所有11个基因的完整ORF序列。两株NSP 3基因均被RCWG确认为一种新的NSP 3基因型19。AzuK-1和Dai-10株的基因型测定显示，除了VP 4、VP 7和NSP 3基因片段外，这两株的8个基因片段具有牛轮状病毒典型的基因型。出乎意料的是，系统发育分析显示AzuK-1和Dai-10株的VP 6和NSP 2基因片段与猴或犬/猫轮状病毒的基因片段聚类，而不是与牛轮状病毒的基因片段聚类。这些研究结果表明，这两种菌株起源于种间传播和多重重配事件涉及牛，猴和犬/猫轮状病毒，导致在引入一些基因到牛轮状病毒的遗传背景的可能性。
We have reported a novel bovine rotavirus, the AzuK-1 (G21P[29]) strain, isolated from an asymptomatic calf. We isolated another bovine rotavirus, the Dai-10 strain, bearing new G24P[33] genotypes, assigned by the Rotavirus Classification Working Group (RCWG), from an asymptomatic cow in Hyogo Prefecture, Japan in 2007. To gain an insight into the origins and evolution of these strains, we determined the complete ORF sequences of all 11 genes of the two strains. The NSP3 genes of both strains were confirmed to belong to a new NSP3 genotype, 19, by the RCWG. Genotype determination of AzuK-1 and Dai-10 strains revealed that eight gene segments of both strains possessed genotypes typically observed in bovine rotaviruses, with the exception of VP4, VP7 and NSP3 gene segments. Unexpectedly, phylogenetic analyses showed that VP6 and NSP2 gene segments of the AzuK-1 and Dai-10 strains were clustered with those of simian or canine/feline rotaviruses, rather than with those of bovine rotaviruses. These findings indicate the possibility that both strains originated by interspecies transmission and multiple reassortment events involving bovine, simian and canine/feline rotaviruses, resulting in the introduction of some genes into the genetic background of bovine rotaviruses.