Harnessing natures ability to control metal ion coordination geometry using de novo designed peptides.
Harnessing natures ability to control metal ion coordination geometry using de novo designed peptides.
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DOI：
10.1039/b818306f
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发表时间：
2009-04-07
期刊：
影响因子：
--
通讯作者：
Pecoraro VL
中科院分区：
文献类型：
--
作者：
Peacock AF;Iranzo O;Pecoraro VL
蛋白质化学、分子和结构生物学的进步使现代化学家能够利用“第一性原理”方法构建复杂的生物结构，这种方法被称为从头开始的蛋白质设计。从这个角度来看，我们证明了简单的三链α-螺旋结构是如何通过单独考虑肽的一级氨基酸序列来制备的。有了这些明确的系统，我们证明了金属结合腔可以从这些聚集体的疏水性核心中雕刻出来，以便结合具有明确配位几何形状的金属离子，如镉。将给出含有这两种物质平衡混合物的同感CdS3复合物、CdS3O位点和蛋白质的例子。我们将提供一种策略的描述，使我们能够构建异色肽（在几乎相同的环境中使两种金属络合的小蛋白质，但导致不同的物理性质并允许金属位点选择性）。最后，我们设计了一类新的肽，二对肽，它可以利用氨基酸手性的变化来控制金属离子配位数，并导致异色体系的另一种途径。本文描述的结构代表制备可能同时包含结构和催化金属结合中心的蛋白质结构的初始步骤。这些研究为生物无机化学的研究提供了新的机遇。
Advances in protein chemistry and molecular and structural biology have empowered modern chemists to build complex biological architectures using a “first principles” approach, which is known as de novo protein design. In this Perspective we demonstrate how simple three-stranded α-helical constructs can be prepared by the sole consideration of the primary amino acid sequence of a peptide. With these well defined systems, we then demonstrate that metal binding cavities can be carved out of the hydrophobic cores of these aggregates in order to bind metal ions such as cadmium with well defined coordination geometries. Examples will be given of homoleptic CdS3 complexes, CdS3O sites and proteins which contain equilibrium mixtures of these two species. We will provide a description of a strategy that allows us to build heterochromic peptides (small proteins that complex two metals in nearly identical environments but which result in different physical properties and allow for metal site selectivity). We conclude with a new class of designed peptides, diastereopeptides, which can exploit changes in amino acid chirality to control metal ion coordination number and lead to an alternative path towards heterochromic systems. The constructs described herein represent the initial steps of preparing protein structures that may simultaneous contain structural and catalytic metal binding centers. These studies inform the community on a developing field, which promises new opportunities for the study of bioinorganic chemistry.
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影响因子：
15
作者：
ARMITAGE, IM;PAJER, RT;COLEMAN, JE
通讯作者：
COLEMAN, JE
影响因子：
15
作者：
Dieckmann, GR;McRorie, DK;Pecoraro, VL
通讯作者：
Pecoraro, VL
影响因子：
56.9
作者：
Dahiyat, BI;Mayo, SL
通讯作者：
Mayo, SL
影响因子：
2.9
作者：
Farrer, BT;Harris, NP;Pecoraro, VL
通讯作者：
Pecoraro, VL
影响因子：
2.9
作者：
Ghosh, D;Lee, KH;Pecoraro, VL
通讯作者：
Pecoraro, VL