Recent advances in the preparation of Fmoc-SPPS-based peptide thioester and its surrogates for NCL-type reactions
Recent advances in the preparation of Fmoc-SPPS-based peptide thioester and its surrogates for NCL-type reactions
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DOI：
10.1007/s11426-016-0381-1
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发表时间：
2017-02
期刊：
影响因子：
--
通讯作者：
Hongxing Li;S. Dong
中科院分区：
文献类型：
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作者：
Hongxing Li;S. Dong
基于Fmoc化学的固相肽合成（SPPS）已经成为肽化学中常用的技术，因为它可以使用自动化机器容易地进行，并且与Boc-SPPS相比不需要高毒性的HF。随着蛋白质化学合成领域的快速发展，人们一直致力于寻找更有效的方法来制备连接反应所需的肽段。本文简要介绍了Fmoc-SPPS衍生肽的工程和修饰的最新进展，这些肽可用作天然化学连接（NCL）或NCL型连接反应中的N端片段。
Solid phase peptide synthesis (SPPS) based on Fmoc chemistry has become a commonly used technique in peptide chemistry, as it can be easily conducted using automated machine, and not requiring highly toxic HF in comparison to Boc-SPPS. With the fast development in the emerging field of protein chemical synthesis, many efforts have been endeavored aiming to find more efficient methods for preparing peptide fragments required in ligation reactions. This review briefly summarizes recent advances in the engineering and modification of Fmoc-SPPS-derived peptides, which can be used as the N-terminal fragments in a native chemical ligation (NCL) or NCL-type ligation reactions.