Plasma MicroRNA 499 as a Biomarker of Acute Myocardial Infarction
Plasma MicroRNA 499 as a Biomarker of Acute Myocardial Infarction
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DOI：
10.1373/clinchem.2010.144121
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发表时间：
2010-07-01
影响因子：
9.3
通讯作者：
Iwai, Naoharu
中科院分区：
文献类型：
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作者：
Adachi, Taichi;Nakanishi, Michio;Iwai, Naoharu
背景：微小RNA（microRNAs，miRNAs）是长度为21-25个核苷酸的内源性小RNA。最近，我们报道了miRNA 208（miR-208）仅在大鼠心肌中产生，并且血浆miR 208是大鼠心肌损伤的生物标志物。在本研究中，我们评估了心肌特异性miRNA的血浆浓度可用于诊断人类心肌损伤的假设。方法：我们使用阵列分析的miRNA生产在各种人体组织中，以确定心脏特异性miRNA。我们评估了14名急性冠状动脉综合征患者、15名充血性心力衰竭患者和10名无心血管疾病患者的miR-499血浆浓度。血浆中miR-499的浓度用实时逆转录PCR方法测定，该方法使用人工小RNA作为内部caliberate.RESULTS：各种人体组织的miRNA阵列分析表明，miR-499几乎只在心脏中产生。血浆miR-499浓度在急性心肌梗死的所有个体中可测量地增加，但低于其他患者组中所有个体的检测限。
BACKGROUND: MicroRNAs (miRNAs) are endogenous small RNAs 21-25 nucleotides in length. Recently, we reported that miRNA 208 (miR-208) is produced exclusively in the rat myocardium and that plasma miR208 is a biomarker of myocardial injury in rats. In the present study, we assessed the hypothesis that plasma concentrations of myocardial-specific miRNAs can be used to diagnose myocardial injury in humans.METHODS: We used array analysis of miRNA production in various human tissues to identify heart-specific miRNAs. We assessed the plasma concentrations of miR-499 in 14 individuals with acute coronary syndromes, 15 individuals with congestive heart failure, and 10 individuals without cardiovascular diseases. Plasma miR-499 concentrations were measured with a real-time reverse-transcription PCR method that used an artificial small RNA as an internal calibrator.RESULTS: The miRNA array analysis of various human tissues indicated that miR-499 was produced almost exclusively in the heart. Plasma miR-499 concentrations were measurably increased in all individuals with acute myocardial infarction but were below the limit of detection for all individuals in the other patient groups.CONCLUSIONS: The plasma concentration of miR-499 may be a useful biomarker of myocardial infarction in humans.